Application of In situ TR Spectroelectrochemical Techniques in Determination of Redox Mechanism of Bioactive Compounds
Sokolová, Romana; Jiroušková, Eliška; Degano, I.; Wantulok, J.; Nycz, J.
2023 - English
The electron transfer reactions play an important role in many natural processes. Reactions such as \ndissociation, protonation, and reactions with water and other small molecules often occur in \nbiotransformation. The reaction schemes involving these chemical reactions coupled to the \nelectron transfer can be determined by cyclic voltammetry. Additionally, in \nsitu spectroelectrochemistry can efficiently contribute to the determination of oxidation or \nreduction mechanism. This technique provides information about the electroactive chromophore during \nthe redox process allowing to identification the first reaction intermediates. The final reaction \nproducts were identified by chromatographic techniques. This study represents a key role of TR \nspectroelectrochemistry in the determination of reaction intermediates in the case of \n1,10- phenanthroline derivative substituted by bioactive phenothiazine unit and \ndrug 3- fluorophenmetrazine (2-(3-fluorophenyl)-3-methylmorpholine, 3-FPM). Co pound 3-FPM\nhave recently appeared as the new psychoactive substance in the drug market.\n
Keywords:
IR spectroelectrochemistry; psychoactive substances; oxidation mechanism
Available on request at various institutes of the ASCR
Application of In situ TR Spectroelectrochemical Techniques in Determination of Redox Mechanism of Bioactive Compounds
The electron transfer reactions play an important role in many natural processes. Reactions such as \ndissociation, protonation, and reactions with water and other small molecules often occur in ...
A polarizable reference electrode
Mareček, Vladimír
2023 - English
New concept of a reference electrode for electrochemical systems resolves a problem of a poor \npotential stability of refence electrodes based on the distribution of a strongly \nhydrophobic common cation between two immiscible electrolyte solutions (TTTES). The use of a \npolarizable metal electrode instead of a classical reference electrode is demonstrated in \n a modified conventional four-electrode cell with TTTES. A simple battery operated potentiostat \ncontrols the working electrode potential in a three-electrode configuration. The working metal \nelectrode then serves as a polarizable reference organic solvent electrode in a four-electrode \nsystem for the polarization of the water/organic solvent interface.\n\n
Keywords:
reference electrode; liquid/liquid interface; chloride extraction
Available on request at various institutes of the ASCR
A polarizable reference electrode
New concept of a reference electrode for electrochemical systems resolves a problem of a poor \npotential stability of refence electrodes based on the distribution of a strongly ...
Determination of Selected Natural Psychoactive Substances in Organic Matrices at 3D Printed Electrodes
Choińska-Mlynarczyk, Marta; Šestáková, Ivana; Navrátil, Tomáš
2023 - English
Psilocybin and its derivative psilocin are popular psychoactive substances both as experimental\ntreatments in clinical trials and as illicit drugs. Their potential ability to influence the human\nbrain entails the need for a fast, inexpensive, selective, and sensitive method of their\ndetermination. The aim of our study was the development of new 3D-printed electrodes which\nfulfill these demands and are useful for medical, toxicological, and forensic purposes.\nSuccessful development allows determining in real matrices (human plasma and dried\nmushrooms) concentrations of the order of 1 μmol dm-3.\n
Keywords:
natural psychoactive substances; 3D-printed electrodes; psilocin; psilocybin
Available on request at various institutes of the ASCR
Determination of Selected Natural Psychoactive Substances in Organic Matrices at 3D Printed Electrodes
Psilocybin and its derivative psilocin are popular psychoactive substances both as experimental\ntreatments in clinical trials and as illicit drugs. Their potential ability to influence the ...
Novel embedding resins for 3D-SEM II
Šlouf, Miroslav; Pavlova, Ewa; Strachota, Beata; Strachota, Adam; Nebesářová, Jana; Týč, Jiří; Krzyžánek, Vladislav; Skoupý, Radim
2023 - English
Research report for Thermo Fisher Scientific s. r. o., based on common project awarded by TA CR (program: National center of competence, TN01000008).
Keywords:
novel embedding resins; 3D-SEM microscopy; higher e-beam resistance
Available at various institutes of the ASCR
Novel embedding resins for 3D-SEM II
Research report for Thermo Fisher Scientific s. r. o., based on common project awarded by TA CR (program: National center of competence, TN01000008).
An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application
Tvorynska, Sofiia; Barek, J.; Josypčuk, Bohdan
2023 - English
An amperometric biosensor consisting of an enzymatic mini-reactor (lactate oxidase covalently\nattached to −NH2 functionalized mesoporous silica powder SBA−15 using glutaraldehyde) and\na silver amalgam-based screen-printed electrode acting as a transducer was developed for the\ndetermination of L-lactic acid (LA) in FIA. The detection potential of −0.9 V vs. Ag pseudoreference\nelectrode was applied for cathodic detection of enzymatically consumed oxygen.\nUnder the optimized conditions, the constructed biosensor enabled selective determination of\nLA with a micromolar limit of detection. Importantly, the proposed biosensor represented\nexcellent operational stability after ≥350 measurements. Finally, it was successfully applied to\nreal sample analysis.
Keywords:
L-lactic acid; Lactate oxidase; Amperometric biosensor; silver amalgam
Available on request at various institutes of the ASCR
An Enzymatic Biosensor with Amperometric Detection in a Flow Injection Analysis for the Determination of L-lactic Acid: Development and Application
An amperometric biosensor consisting of an enzymatic mini-reactor (lactate oxidase covalently\nattached to −NH2 functionalized mesoporous silica powder SBA−15 using glutaraldehyde) and\na silver ...
3D Printed Microfluidic Cell for Spectroelectrochemical Analysis
Šikula, M.; Vaněčková, Eva; Hromadová, Magdaléna; Kolivoška, Viliam
2023 - English
Recent advances in fused deposition modeling 3D printing (FDM 3DP) enabled \nthe manufacture of customized spectroelectrochemical (SEC) devices. Despite significant progress, \nreported designs still rely on conventional optical components (windows and cuvettes). In this \nwork, we apply bi-material FDM 3DP combining electrically conductive and optically \ntranslucent filaments to manufacture a fully integrated microfluidic SEC device. Employing cyclic \nvoltammetric measurements with redox probes, we demonstrate that the platform allows SEC sensing of \nreactants, intermediates, and products of charge transfer reactions in an oxygen- free \nenvironment. Developed approaches pave the way for SEC d vices with dramatically\nreduced costs compared to currently available commercial platforms.\n
Keywords:
spectroelectrochemistry; three-dimensional printing; fused deposition modeling
Available on request at various institutes of the ASCR
3D Printed Microfluidic Cell for Spectroelectrochemical Analysis
Recent advances in fused deposition modeling 3D printing (FDM 3DP) enabled \nthe manufacture of customized spectroelectrochemical (SEC) devices. Despite significant progress, ...
Electrochemical Properties of Mixed Self-Assembled Monolayers Based on Terpyridine Transition Metal Complexes
Kocábová, Jana; Nováková Lachmanová, Štěpánka; Vavrek, František; Valášek, M.; Hromadová, Magdaléna
2023 - English
This work presents the electrochemical characterization of mixed self-assembled monolayers\n(SAMs) of [Os(terpy)2]2+ and terpyridine connected by tripodal anchoring groups to the\npolycrystalline gold bead electrode and provides a comparison with SAM based on\n[Os(terpy)2]2+ tripodal molecules without diluting terpyridine tripodal molecules. It is shown\nthat both arrangements enable reproducible preparation of well-defined compact films. The\nelectron transfer rate constant for mixed SAM was determined from cyclic voltammograms\naccording to Laviron’s analysis and compared with the rate constant obtained previously for\nSAM containing only [Os(terpy)2]2+ tripod.
Keywords:
transition metal complexes; terpyridine; tripodal anchor
Available on request at various institutes of the ASCR
Electrochemical Properties of Mixed Self-Assembled Monolayers Based on Terpyridine Transition Metal Complexes
This work presents the electrochemical characterization of mixed self-assembled monolayers\n(SAMs) of [Os(terpy)2]2+ and terpyridine connected by tripodal anchoring groups to the\npolycrystalline gold ...
Elimination Voltammetry with Linear Scan: Advantages and Disadvantages
Navrátil, Tomáš; Trnková, L.; Hrdlička, Vojtěch; Li, X.
2023 - English
Elimination voltammetry with linear scan (EVLS) represents an almost traditional\nmathematical method that helps to obtain additional and detailed information about the recorded\nelectrochemical signals and therefore, to better understand the analyzed systems. Although it\nhas been substantially developed and expanded during its existence of more than a quarter of a\ncentury and has found many areas of application, like any other mathematical and experimental\nmethod, it exhibits several advantages and disadvantages. In this contribution, we summarize\nits strengths, weaknesses, opportunities, as well as threats.\n
Keywords:
elimination voltammetry with linear scan; advantages; disadvantages
Available on request at various institutes of the ASCR
Elimination Voltammetry with Linear Scan: Advantages and Disadvantages
Elimination voltammetry with linear scan (EVLS) represents an almost traditional\nmathematical method that helps to obtain additional and detailed information about the recorded\nelectrochemical ...
4th CCP Phenogenomics Conference abstract book
Sedláček, Radislav
2022 - English
The fourth CCP Phenogenomics Conference was held as a hybrid meeting. The scientific committee selected the topic of rare diseases: experimental models & delivery of therapies as the main thematic focus of the 2022 Conference. The Conference provided again an excellent opportunity to support networking and interactions among the researchers, CCP staff, users and experts from the commercial sector.
Keywords:
phenogenomics; rare diseases; gene therapy; experimental models; delivery of therapies
Available at various institutes of the ASCR
4th CCP Phenogenomics Conference abstract book
The fourth CCP Phenogenomics Conference was held as a hybrid meeting. The scientific committee selected the topic of rare diseases: experimental models & delivery of therapies as the main thematic ...
Novel embedding resins for 3D-SEM
Šlouf, Miroslav; Pavlova, Ewa; Strachota, Beata; Strachota, Adam; Nebesářová, Jana; Týč, Jiří; Krzyžánek, Vladislav; Skoupý, Radim
2022 - English
The report contains the detailed analysis of novel embedding resins for 3D-SEM.
Keywords:
novel embedding resins; 3D-SEM microscopy; higher e-beam resistance
Available at various institutes of the ASCR
Novel embedding resins for 3D-SEM
The report contains the detailed analysis of novel embedding resins for 3D-SEM.
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