Number of found documents: 47
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Effect of exposure to bisphenol A on gene expression in the testicular tissue in male mice
Dorosh, Andriy; Elzeinová, Fatima; Žatecká, Eva; Kubátová, Alena; Pěknicová, Jana
2014 - English
Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic Bisphenol A (BPA) is a synthetic, endocrine-disrupting compound able to directly bind estrogen receptors. Free BPA has been detected in human samples indicating that humans are internally exposed to BPA. The purpose of this study was to analyse the effect of BPA on the male reproductive system and testicular gene expression in germ cells. We studied the influence of long-term low concentration BPA exposition on male fertility in vivo in a two-generation study in C57BL/6 mouse strain. In this work, BPA was added with water at two environmentally relevant concentrations: 0, 4 and 4 µg/l. We measured the reproductive organs weight and sperm cells morphology and quality. Expression of genes involved in endocrine regulation and energy metabolism in testis was analysed after BPA exposure. Next, the epigenetic mechanisms of gene expression and regulation during the germ cell differentiation and effect of BPA will be analysed. Keywords: bisphenol A; gene expression; testicular tissue Available at various institutes of the ASCR
Effect of exposure to bisphenol A on gene expression in the testicular tissue in male mice

Laboratory of Reproductive Biology, Institute of Biotechnology, Academy of Sciences of the Czech Republic, v.v.i., Prague, Czech Republic Bisphenol A (BPA) is a synthetic, endocrine-disrupting ...

Dorosh, Andriy; Elzeinová, Fatima; Žatecká, Eva; Kubátová, Alena; Pěknicová, Jana
Biotechnologický ústav, 2014

Expression of estrogen receptor beta (ERβ) In murine male reproductive tract and sperm
Dostálová, Pavla; Děd, Lukáš; Dorosh, Andriy; Elzeinová, Fatima; Pěknicová, Jana
2014 - English
Estrogens are steroid hormones that play an important role in reproduction of both sexes. In male, the main source of estrogens are testes where both somatic and germ cells are responsible for testosteron conversion to estrogens. Estrogens are involved in control of spermatogenesis, fluid reabsorption in rete testis and epididymis, and in later maturation steps that sperm undergo in female genital tract (capacitation, acrosome reaction). Generally, estrogen action is mediated through binding to estrogen receptors (ERs) which than lead to classical genomic or rapid non-genomic signaling. Nowadays, two classical estrogen receptors are known – ERα and ERβ. ERβ is a predominant variant in testes, while ERα is more abundant in rete testis and initial segment of epididymis. In addition to classical ERs, several splice variants that can differ in their ligand- or DNA-binding properties were detected in different tissues and cell lines. ERs mostly work as a dimer (homo- and hetero-) and splice variants often „only“ modulate function of classical full-length ERs. Therefore, estrogen action seems to be a very complex. To contribute to understanding of estrogen action in male, we detected ERβ and its potential splice variants in mice testis, epididymis and sperm. According to our results, two variants are present in all analysed tissues and cells. These variants differ in one exon in ligand binding domain which leads to different affinity for estrogens. To analyse these variants also at a protein level, we prepared specific monoclonal antibodies recognizing particular variant of ERβ. Both atibodies detected band(s) in protein extracts from testes or epididymis. Taking together, there are at least two variants of ERβ in mice testes, epididymis and sperm and it seems that both variants are similar in abundance within the same organ or sperm. Keywords: estrogene receptor beta; spermatozoa; splice variant Available at various institutes of the ASCR
Expression of estrogen receptor beta (ERβ) In murine male reproductive tract and sperm

Estrogens are steroid hormones that play an important role in reproduction of both sexes. In male, the main source of estrogens are testes where both somatic and germ cells are responsible for ...

Dostálová, Pavla; Děd, Lukáš; Dorosh, Andriy; Elzeinová, Fatima; Pěknicová, Jana
Biotechnologický ústav, 2014

Detection of mannosidase in the porcine urogenital tract – study of the sperm releasing from oviductal reservoir
Maňásková, Pavla; Ren, Š.; Jelínková, Jitka; Krejčová, T.; Liberda, J.
2014 - English
One of the most important steps of reproduction process is the meeting of sperm with oocyte. Binding of sperm with oviductal cells maintains spermatozoa in fertile state. The beginning of sperm capacitation is associated with oocyte ovulation resulting in the sperm release from oviductal reservoir. Hormonal changes after ovulation probably induce distinct oviductal secretion leading to disruption of the sperm protein binding with oviductal saccharide moieties. Another eventuality of the sperm releasing from oviductal reservoir is a change of oviductal environment caused by components of follicular fluid transported with oocyte after ovulation. In the pig, previous studies indicate lectin-type interaction of sperm protein receptors by mannose structures on the surface of oviductal cells. Our study was focused on enzymatic activity of mannosidase and its detection in porcine oviduct (fluid and tissue) and follicular fluid during hormonal cycle. In fluid from follicles in early and late hormonal stages, we measured mannosidase activity by colorimetric methods at physiological and acidic pH. Expression of secreted mannosidase was studied by specific antibody in follicular and oviductal fluids, and oviductal tissues during hormonal cycle. Clearly increased enzymatic activity of secreted mannosidase was found as specific-species in porcine fluid from follicles in late stage of hormonal cycle. On the other hand, detection of secreted mannosidase in follicular fluid as well as in oviductal fluid did not shown any significant differences during hormonal cycle. In oviductal isthmic tissue, we detected decreased protein expression of secreted mannosidase at middle and late follicular phases. These results suggest possible role of follicular mannosidase rather than oviductal one in the sperm releasing from oviductal reservoir in the pig. The additional study of the gene expression of secreted form of mannosidase in oviductal tissue during hormonal cycle should be necessary. Keywords: mannosidase; oviductal reservoir; follicular fluid; enzymatic activity Available at various institutes of the ASCR
Detection of mannosidase in the porcine urogenital tract – study of the sperm releasing from oviductal reservoir

One of the most important steps of reproduction process is the meeting of sperm with oocyte. Binding of sperm with oviductal cells maintains spermatozoa in fertile state. The beginning of sperm ...

Maňásková, Pavla; Ren, Š.; Jelínková, Jitka; Krejčová, T.; Liberda, J.
Biotechnologický ústav, 2014

Konferenční sborník XVIII. symposium českých reprodukčních imunologů s mezinárodní účastí
Pěknicová, Jana; Elzeinová, Fatima; Kubátová, Alena
2012 - English
Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction. Keywords: immunology of reproduction; autoimmune diseases; antibodies; spermatozoa; oocytes; proteins; endocrine disruptors Available at various institutes of the ASCR
Konferenční sborník XVIII. symposium českých reprodukčních imunologů s mezinárodní účastí

Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction.

Pěknicová, Jana; Elzeinová, Fatima; Kubátová, Alena
Biotechnologický ústav, 2012

METODICKÉ DOPORUČENÍ PRO VYKAZOVÁNÍ SKUTEČNÝCH ZPŮSOBILÝCH NEPŘÍMÝCH NÁKLADŮ NA PROJEKT PRO ÚSTAVY AV ČR
Škoda, Jan; Starosta, Karel
2012 - Czech
Metodické doporučení si klade za cíl formulovat společné možné zásady pro pracoviště AV ČR týkající se alokace nepřímých nákladů na projekty a to především s ohledem na pravidla poskytovatelů, kteří přiřazování nepřímých nákladů metodou full cost vyžadují. Metodika vychází z obvyklých hospodářských a účetních postupů ústavů AV ČR. Navrhované řešení je založeno na maximální jednoduchosti a efektivnosti s cílem minimálního navýšení nepřímých nákladů zapříčiněných užíváním této metodiky, umožňuje zakončení hospodářského roku v řádném termínu a vykázání potřebných dat poskytovatelům. The aim of the Methodical Recommendation is to create possible common principles for institutes of the Academy of Sciences of the Czech Republic (AS CR) concerning the allocation of indirect costs to projects, considering mainly the rules of fund providers requesting reporting of real costs via full cost methodology. Proposed methodology is based on usual management and accounting practices of institutes of AS CR and lie in principles of maximum simplicity and effectiveness. It should result in minimum increase of real indirect costs caused by application of such methodology, it enables closing of economic year on time and also reporting of necessary data to fund providers. Keywords: AS CR; overheads; indirect costs; full cost Available at various institutes of the ASCR
METODICKÉ DOPORUČENÍ PRO VYKAZOVÁNÍ SKUTEČNÝCH ZPŮSOBILÝCH NEPŘÍMÝCH NÁKLADŮ NA PROJEKT PRO ÚSTAVY AV ČR

Metodické doporučení si klade za cíl formulovat společné možné zásady pro pracoviště AV ČR týkající se alokace nepřímých nákladů na projekty a to především s ohledem na pravidla poskytovatelů, kteří ...

Škoda, Jan; Starosta, Karel
Biotechnologický ústav, 2012

Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation
Pěknicová, Jana; Elzeinová, Fatima; Cozlová, Nina
2011 - English
Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction. Keywords: immunology of reproduction; autoimmune diseases; antibodies; spermatozoa; oocytes; proteins; endocrine disruptors Available at various institutes of the ASCR
Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation

Book of abstract XVIIth Symposium of Czech Reproductive Immunologist with international participation contains abstracts of lectures which focuse on immunology and physiology of reproduction.

Pěknicová, Jana; Elzeinová, Fatima; Cozlová, Nina
Biotechnologický ústav, 2011

Vliv endokrinních disruptorů na reprodukci savců
Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Boubelík, Michael; Elzeinová, Fatima; Kubátová, Alena
2008 - Czech
Endocrine system is one of the most important regulatory systems in the mammalian body. The endocrine disruptors (ED) can disrupt the function of the endocrine system but their activity is not a result of the physiological regulation. The effect of low dose of selected ED, bisphenol-A (BPA), p-nonylphenol (NP), diethylstilbestrol (DES), genistein (GEN), resveratrol (RES)), hormone (17α-ethynylestradiol (EE2)) and polybrominated compound (tetrabromobisphenol A (TBBPA)) within development of reproductive tract in outbred CD1 mice was tested. BPA and NP had a negative effect on reproductive organs morphology and mouse spermatozoa. The fertility of mice exposed to DES was disrupted. Contrary to that, treatment by GEN and RES had no effect on the fertility of mice but had some effect on their reproductive parameters except for sperm integrity. Synthetic hormone (EE2) and polybrominated compound (TBBPA) also affected on the organ weight, reproductive parameters and sperm quality. Keywords: endocrine disruptor; xenoestrogen; phytoestrogen; sperm integrity; endokrinní systém; plodnost Available at various institutes of the ASCR
Vliv endokrinních disruptorů na reprodukci savců

Endocrine system is one of the most important regulatory systems in the mammalian body. The endocrine disruptors (ED) can disrupt the function of the endocrine system but their activity is not a ...

Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Boubelík, Michael; Elzeinová, Fatima; Kubátová, Alena
Biotechnologický ústav, 2008

The effect of low dose of vinclozolin on reproductive tract development in CD1 outbred mice
Elzeinová, Fatima; Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Kubátová, Alena
2008 - English
The effect of a low dose of vinclozolin within the development of the reproductive tract during gestation (VIN-GD 15-22) and puberty (VIN-PND 23-44) in CD1 mice was tested. We found a decrease in the anogenital distance, prostate weight and pathology of testes in both experimental groups. Sperm counts decreased to 46% (VIN-GD) and to 81% (VIN-PND), and also the acrosomal state (evaluated by antiacrosomal antibody) decreased in both groups to 89% in comparison to the control group (100%). Sperm head abnormalities increased by approximately 18% and 13%, respectively. In this connection, the expression of some genes was changed (arosome-related gene (Acr), apoptosis related genes (p53, p21)). In conclusion, a low dose of vinclozolin affected the reproductive tract, sperm parameters and expression of selected genes in both experimental groups. Keywords: vinclozolin; CD1 mouse; spermatozoa; geny; teorie grup Available at various institutes of the ASCR
The effect of low dose of vinclozolin on reproductive tract development in CD1 outbred mice

The effect of a low dose of vinclozolin within the development of the reproductive tract during gestation (VIN-GD 15-22) and puberty (VIN-PND 23-44) in CD1 mice was tested. We found a decrease in the ...

Elzeinová, Fatima; Pěknicová, Jana; Nováková, Vendula; Buckiová, Daniela; Kubátová, Alena
Biotechnologický ústav, 2008

Monoklonální protilátky rozpoznávající proteiny lidské semenné plazmy
Čapková, Jana; Elzeinová, Fatima; Margaryan, Hasmik; Koubek, Pavel; Pěknicová, Jana
2008 - Czech
Ustavili jsme panel monoklonálních protilátek protil lidským spermiím (Hs- serie, Praha) a charakterizovali jsme je pomocí imunofluorescence, Western bloting techniky a MALDI analýzy. V našem panelu Hs- protilátek několik protilátek reagovalo s proteiny lidské semenné plasmy. Tyto protilátky mohou být užitečné pro studium kapacitace spermií a hodnocení kvality spermatu. We have generated a set of monoclonal antibodies to human spermatozoa (Hs- serie, Prague) and characterized them using immunofluorescence, Western bloting and MALDI analysis. In our panel of Hs- antibodies several antibodies specifically reacted with human seminal plasma proteins. These antibodies might be useful for study sperm capacitation and sperm quality assessment. Keywords: monoclonal antibody; seminal plasma; spermatozoa Available at various institutes of the ASCR
Monoklonální protilátky rozpoznávající proteiny lidské semenné plazmy

Ustavili jsme panel monoklonálních protilátek protil lidským spermiím (Hs- serie, Praha) a charakterizovali jsme je pomocí imunofluorescence, Western bloting techniky a MALDI analýzy. V našem panelu ...

Čapková, Jana; Elzeinová, Fatima; Margaryan, Hasmik; Koubek, Pavel; Pěknicová, Jana
Biotechnologický ústav, 2008

Differences in the strategies of mammalian and fish fertilization and the optimization of the protocol for immunofluorescence on fish spermatozoa
Koubek, Pavel; Králová, Alena; Pšenička, M.; Pěknicová, Jana
2008 - English
This lecture describes a special approach to handling fish spermatozoa in comparison with the symplex originating from mammals. This approach primarily emphasizes the concrete parts of immunofluorescence protocol determining accurate results. Vzhledem ke struktuře rybích spermií, která je jednoduchá a snadno poškoditelná, bylo třeba vypracovat protokol pro imunofluorescenční značení, který by minimalizoval jejich poškození a vznik nespecifických artefaktů. Cílem práce tak bylo především zamezit mylné interpretaci výsledků imunofluorescenčních experimentů na rybích spermiích a zvýšit vzájemnou porovnatelnost těchto experimentů. Keywords: fish spermatozoa; mammalian spermatozoa; immunofluorescence Available at various institutes of the ASCR
Differences in the strategies of mammalian and fish fertilization and the optimization of the protocol for immunofluorescence on fish spermatozoa

This lecture describes a special approach to handling fish spermatozoa in comparison with the symplex originating from mammals. This approach primarily emphasizes the concrete parts of ...

Koubek, Pavel; Králová, Alena; Pšenička, M.; Pěknicová, Jana
Biotechnologický ústav, 2008

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