Název:
Utilization of micropreparative fast focusing by a new wide pH range electrolyte system based on bidirectional isotachophoresis
Autoři:
Vykydalová, Marie ; Duša, Filip ; Horká, Marie ; Šlais, Karel Typ dokumentu: Příspěvky z konference Konference/Akce: CECE Junior 2013, Brno (CZ), 2013-11-12 / 2013-11-13
Rok:
2013
Jazyk:
eng
Abstrakt: We suggest the possibility of practical utilization of a new electrolyte system for fast preparative focusing in wide pH range based on bidirectional isotachophoresis. The focusing occurs on nonwoven fabric strip positioned in an open horizontal V-shaped trough. It is based on bidirectional ITP with multiple counter ions and spacers created from commercially available simple buffers. Milk spiked with ampicillin was used as a sample and high performance liquid chromatography was as a second dimension for analysis of fast preparative focusing fractions. Speed, easy fraction handling, and possibility of pre-concentration of analytes from a raw sample are the benefits of this technique.
Klíčová slova:
bidirectional isotachophoresis; electrolyte system; preparative focusing Číslo projektu: VG20112015021 (CEP) Poskytovatel projektu: GA MV Zdrojový dokument: Chemické Listy. Roč. 107, Issue s3., ISSN 0009-2770
Instituce: Ústav analytické chemie AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný na vyžádání prostřednictvím repozitáře Akademie věd. Původní záznam: http://hdl.handle.net/11104/0229245