Název:
Physical and biocatalytic properties of polyvinyl alcohol lens-shaped particles versus spherical polyelectrolyte complex microcapsules
Autoři:
Schenkmayerová, A. ; Bučko, M. ; Gemeiner, P. ; Treľová, D. ; Lacík, I. ; Chorvát Jr., D. ; Ačai, P. ; Polakovič, M. ; Lipták, L. ; Rebroš, M. ; Rosenberg, M. ; Štefuca, V. ; Neděla, Vilém ; Tihlaříková, Eva Typ dokumentu: Příspěvky z konference Konference/Akce: Workshop of Interesting Topics of SEM and ESEM, Mikulov (CZ), 2014-08-26 / 2014-08-31
Rok:
2014
Jazyk:
eng
Abstrakt: Direct comparison of key physical and chemical-engineering properties of two representative matrices for multipurpose immobilisations was performed for the first time. Polyvinyl alcohol lens-shaped particles LentiKats and polyelectrolyte complex microcapsules were characterised by advanced techniques with respect to the size distribution of the particles, their inner morphology as revealed by fluorescent probe staining, mechanical resistance, size-exclusion properties, determination of effective diffusion coefficient and environmental scanning electron microscope imaging. Recombinant cells E. coli overexpressing enzyme cyclopentanone monooxygenase were immobilised in polyelectrolyte complex microcapsules and LentiKats for comparison of their operational stability using model Baeyer-Villiger oxidation of (±)-cis-bicyclo[3.2.0]hept-2-en-6-one to regioisomeric lactones as important chiral synthons for potential pharmaceuticals.
Klíčová slova:
ESEM; SEM Číslo projektu: EE.2.3.20.0103 Poskytovatel projektu: GA MŠk Zdrojový dokument: Workshop of Interesting Topics of SEM and ESEM, ISBN 978-80-87441-12-1
Instituce: Ústav přístrojové techniky AV ČR
(web)
Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0238653