Název:
Effect of H- and O-termination of nanocrystalline diamond films on cell adhesion and osteogenic differentiation
Autoři:
Lišková, Jana ; Babchenko, Oleg ; Varga, Marián ; Kromka, Alexander ; Hadraba, Daniel ; Švindrych, Zdeněk ; Bačáková, Lucie Typ dokumentu: Příspěvky z konference Konference/Akce: International Conference NANOCON /6./, Brno (CZ), 2014-11-05 / 2014-11-07
Rok:
2014
Jazyk:
eng
Abstrakt: In order to control the hydrophobic or hydrophilic character, the NCD film surfaces were grafted by hydrogen atoms (H-termination) or by oxygen atoms (O-termination). Human mesenchymal stem cells and primary human osteoblasts were used for biological studies on H- and O-terminated NCD films. We found that cells cultivated on O-terminated NCD films exhibit better adhesion than H-terminated NCD films. In addition, the expression of osteogenic cell markers collagen and alkaline phosphatase analysed by Realtime PCR and immunostaining was higher on O-terminated films.
Klíčová slova:
MSC; nanocrystalline diamond film; osteoblast; paxillin; type I collagen Číslo projektu: GA14-04790S (CEP), EE2.3.30.0025, ED1.1.00/02.0109, CZ.1.05/1.1.00/02.0109 Poskytovatel projektu: GA ČR, GA MŠk, GA MŠk, MSMT Zdrojový dokument: NANOCON 2014 Conference Proceedings, ISBN 978-80-87294-55-0
Instituce: Fyzikální ústav AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0243399