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Název:
Vliv environmentálních stresů na mutabilitu u Bacillus subtilis - role mismatch-repair systému
Překlad názvu: Effect of environmental stresses on mutability of Bacillus subtilis - role of mismatch-repair system
Autoři: Nunvář, Jaroslav ; Lichá, Irena (vedoucí práce) ; Konopásek, Ivo (oponent)
Typ dokumentu: Diplomové práce
Rok: 2009
Jazyk: cze
Abstrakt: The everchanging nature of bacterial environment requires adaptation to emerging novel conditions. One proposed way of adaptation involves increased generation of genetic variability in response to harmful conditions - a phenomenon called adaptive mutagenesis. However, the details of mechanisms of adaptive mutagenesis, and even its very existence, are far from clear. Our goal was to subject the Gram-positive model bacterium Bacillus subtilis to variety of environmental stresses, examine the rate of mutagenesis occuring and compare it to unstressed conditions. Next we wondered if there was a role for mismatch-repair system (MMR), the major pathway for mutation avoidance, in these processes. To accomplish this, we constructed systems to monitor the expression of MMR components both on transcription and translation level. We also developed a mathematical model for precise mutation rate determination in order to quantify the intensity of mutagenic processes. The monitoring of MMR proteins translation failed due to high background endogenous fluorescence present in B. subtilis cells. However, we found out, using transcription reporter system, that the expression of MMR is not influenced by imposition of harsh hyperosmotic shock upon cells. The expression of MMR was also barely influenced by nutrient limitation...
Instituce: Fakulty UK (VŠKP) (web)
Informace o dostupnosti dokumentu: Dostupné v digitálním repozitáři UK.
Původní záznam: http://hdl.handle.net/20.500.11956/26183
Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-281272
Záznam je zařazen do těchto sbírek:
Školství > Veřejné vysoké školy > Univerzita Karlova > Fakulty UK (VŠKP)
Vysokoškolské kvalifikační práce > Diplomové práce
Překlad názvu: Effect of environmental stresses on mutability of Bacillus subtilis - role of mismatch-repair system
Autoři: Nunvář, Jaroslav ; Lichá, Irena (vedoucí práce) ; Konopásek, Ivo (oponent)
Typ dokumentu: Diplomové práce
Rok: 2009
Jazyk: cze
Abstrakt: The everchanging nature of bacterial environment requires adaptation to emerging novel conditions. One proposed way of adaptation involves increased generation of genetic variability in response to harmful conditions - a phenomenon called adaptive mutagenesis. However, the details of mechanisms of adaptive mutagenesis, and even its very existence, are far from clear. Our goal was to subject the Gram-positive model bacterium Bacillus subtilis to variety of environmental stresses, examine the rate of mutagenesis occuring and compare it to unstressed conditions. Next we wondered if there was a role for mismatch-repair system (MMR), the major pathway for mutation avoidance, in these processes. To accomplish this, we constructed systems to monitor the expression of MMR components both on transcription and translation level. We also developed a mathematical model for precise mutation rate determination in order to quantify the intensity of mutagenic processes. The monitoring of MMR proteins translation failed due to high background endogenous fluorescence present in B. subtilis cells. However, we found out, using transcription reporter system, that the expression of MMR is not influenced by imposition of harsh hyperosmotic shock upon cells. The expression of MMR was also barely influenced by nutrient limitation...
Instituce: Fakulty UK (VŠKP) (web)
Informace o dostupnosti dokumentu: Dostupné v digitálním repozitáři UK.
Původní záznam: http://hdl.handle.net/20.500.11956/26183
Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-281272
Záznam je zařazen do těchto sbírek:
Školství > Veřejné vysoké školy > Univerzita Karlova > Fakulty UK (VŠKP)
Vysokoškolské kvalifikační práce > Diplomové práce
Záznam vytvořen dne 2017-04-25, naposledy upraven 2022-03-03.