Inihibice glukuronidace léčiv extrakty a obsahovými látkami třezalky tečkované (Hypericum perforatum) a tymiánu obecného (Thymus vulgaris)

Čejchanová, Jana

Charles University in Prague Julius-Maximilians-University Würzburg Fac. of Pharmacy in Hradec Králové Institute of Pharmacy and Food Chemistry Dept. of Biochemical Sciences Dept. of Pharmaceutical Chemistry Candidate: Jana Čejchanová Supervisor: PharmDr. Iva Boušová, Ph.D. PD Dr. Matthias Unger Title of diploma thesis: Inhibition of Drug Glucuronidation by Extracts and Constituents of St. John's wort (Hypericum perforatum) and Thyme (Thymus vulgaris) The issue of herb-drug interactions is discussed in last years more than ever before due to all over the world increasing popularity of herbal drugs. Whereas the awareness of herbal drugs interactions with cytochrome P450 is quite broad, the issue of their interaction with UDP-glucuronosyltransferase (UGT) enzymes, one of the main participants of the second phase metabolism in humans, is still full of questions. This work is focused on medicinal herbs St. John's wort (Hypericum perforatum) and thyme (Thymus vulgaris) and their impact on glucuronidation in the in vitro studies. The HPLC method for fractionation of herbal extracts was developed. The inhibitory activity of herbal extracts, individual fractions, and selected constituents on UGT was studied in human liver and/or human intestinal microsomes using model substrate TFMU...

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