Název: In vivo study of diatom assemblages using low temperature method for ESEM
Autoři: Tihlaříková, Eva ; Neděla, Vilém ; Fránková, Markéta
Typ dokumentu: Příspěvky z konference
Konference/Akce: Multinational Congress on Microscopy /13./, Rovinj (HR), 20170924
Rok: 2017
Jazyk: eng
Abstrakt: Diatoms are the most species-rich algal group represented by 12 000 described species and are recognized as powerful bio indicators and used for water quality monitoring. Diatom taxonomy is predominantly based on the morphology of ornate silicified cell wall called frustule composed of two overlapping parts (thecae). This frustule displays intricate patterns and designs unique to each species. For these studies conventional scanning electron microscopy (SEM) was and still is widely used. This method requires cleaning of diatom frustules in strong acids and peroxides followed by conductive coating.This aggressive procedure removes protoplast and damages delicate structures. Environmental scanning electron microscopy (ESEM) brings advantages of observation of fresh diatom material that are presence of whole intact diatom cells, not only empty diatom frustules, extracellular mucilaginous diatom secrets (e.g. pads, stalks, tubes) and whole diatom assemblages directly in situ together with other algal assemblages (e.g. cyanobacteria or green algae). However, in diatom research, ESEM is used mainly for elimination of conductive coating and observation of these organisms in their native wet state adhered directly on the host plant is not yet common. The ESEM observation of native aquatic samples is usually affected with radiation damage that can be lovered by the use of special methods like the Low Temperature Method (LTM) for the ESEM.
Klíčová slova: diatom; ESEM; LTM; morphology
Číslo projektu: LO1212 (CEP), ED0017/01/01
Poskytovatel projektu: GA MŠk, GA MŠk
Zdrojový dokument: 13th Multinational Congress on Microscopy: Book of Abstracts, ISBN 978-953-7941-19-2

Instituce: Ústav přístrojové techniky AV ČR (web)
Informace o dostupnosti dokumentu: Dokument je dostupný v příslušném ústavu Akademie věd ČR.
Původní záznam: http://hdl.handle.net/11104/0277119

Trvalý odkaz NUŠL: http://www.nusl.cz/ntk/nusl-373316


Záznam je zařazen do těchto sbírek:
Věda a výzkum > AV ČR > Ústav přístrojové techniky
Konferenční materiály > Příspěvky z konference