Rozpoznávanie nového kolonizačného faktora enterotoxigennej E. coli CS30 sacharidmi

Chrienová, Žofia

Charles University Faculty of Pharmacy in Hradec Králové Department of Pharmaceutical Chemistry and Pharmaceutical Analysis University of Gothenburg Sahlgrenska Academy Institute of Biomedicine, Department of Medicinal Biochemistry and Cell Biology, Department of Microbiology and Immunology Student: Žofia Chrienová Supervisors: PharmDr. Marta Kučerová, Ph.D. Prof. Susann Teneberg, MD PhD Astrid von Mentzer, PhD Title of diploma thesis: Carbohydrate recognition of the novel colonization factor CS30 of enterotoxigenic E. coli Enterotoxigenic Escherichia coli (ETEC) is a major cause of diarrhoea in developing countries and travellers to these areas, as well as in many farm animals. The main ETEC virulence factors are two enterotoxins and outer membrane proteins called colonization factors that mediate the adherence of the bacteria to the host epithelial cell surface in the small intestine. Carbohydrate recognition of the recently identified colonization factor CS30 was characterised using binding studies with the mixture of glycosphingolipids isolated from the human and porcine intestine. The CS30 binding compound was identified as a mixture of sulfatides with different ceramide species by mass spectrometry and a binding assay with monoclonal antibodies directed towards SO3-3Galβ. Further testing...

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