Název:
Rapid fabrication of microextraction devices for direct determination of basic drugs in dried blood spots
Autoři:
Ryšavá, Lenka ; Přikryl, Jan ; Dvořák, Miloš ; Malá, Zdeňka ; Kubáň, Pavel Typ dokumentu: Příspěvky z konference Konference/Akce: CECE 2018. International Interdisciplinary Meeting on Bioanalysis /15./, Brno (CZ), 20181015
Rok:
2018
Jazyk:
eng
Abstrakt: A 3D-printed cutter was designed for rapid fabrication of supported liquid membrane (SLM) extraction devices compatible with injection system of commercial capillary electrophoresis (CE) instrument. Low cost and single use microextraction devices were fabricated from polypropylene micropipette tips with high precision (dimension variances ≤ 3% RSD). Practical applicability of the microextraction devices was examined by in-line coupling of SLM extractions to CE for direct analysis of four model basic drugs in dried blood spot extracts. Excellent repeatability of the hyphenated technique was achieved (RSD ≤ 7.6%), transfer of analytes across the SLM ranged from 23 to 73% and limits of detection were as low as 0.07 μg/mL.
Klíčová slova:
capillary electrophoresis; microextraction devices; supported liquid membrane (SLM) Číslo projektu: GA18-13135S (CEP) Poskytovatel projektu: GA ČR Zdrojový dokument: CECE 2018. 15th International Interdisciplinary Meeting on Bioanalysis, ISBN 978-80-904959-5-1