Název:
Synthesis of biaryl-substituted fluorescent nucleosides and nucleoside triphosphates and their incorporation to DNA
Autoři:
Riedl, Jan ; Hocek, Michal Typ dokumentu: Příspěvky z konference Konference/Akce: Chemistry of Nucleic Acid Components /15./, Český Krumlov (CZ), 2011-06-05 / 2011-06-10
Rok:
2011
Jazyk:
eng
Abstrakt: A series of modified nucleosides bearing the fluorescent substituted biaryl labels attached to nucleobase was prepared and their luminescent properties were evaluated. The modified nucleosides show divers fluorescence from 400 to 560 nm when excited at 340 nm in water depending on structural and substitution pattern. The corresponding biaryl-substituted nucleoside triphosphates (dNTPs) were prepared analogously and used for polymerase incorporation to DNA. Applications in hybridization probes were studied.
Klíčová slova:
DNA; fluorescence; nucleotides Číslo projektu: CEZ:AV0Z40550506 (CEP), LC512 (CEP), GA203/09/0317 (CEP) Poskytovatel projektu: GA MŠk, GA ČR Zdrojový dokument: Chemistry of Nucleic Acid Components. 15th Symposium, ISBN 978-80-86241-37-1
Instituce: Ústav organické chemie a biochemie AV ČR
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Informace o dostupnosti dokumentu:
Dokument je dostupný v příslušném ústavu Akademie věd ČR. Původní záznam: http://hdl.handle.net/11104/0198829