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Maximizing efficiency of field-amplified electrokinetic injection of weak bases for enantioselective capillary electrophoresis with sulfated cyclodextins
Šesták, Jozef; Thormann, W.
2018 - English
The optimal conditions for field-amplified electrokinetic injection of cations followed by their separation based on the stereoselective interaction with sulfated cyclodextrins are discussed. Results show that addition of a short water plug together with a short plug free of cyclodextrins and with a buffer concentration similar to the BGE greatly increase the injection efficiency.
Keywords:
capillary electrophoresis; cyclodextrins
Fulltext is available at external website.
Maximizing efficiency of field-amplified electrokinetic injection of weak bases for enantioselective capillary electrophoresis with sulfated cyclodextins
The optimal conditions for field-amplified electrokinetic injection of cations followed by their separation based on the stereoselective interaction with sulfated cyclodextrins are discussed. Results ...
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells
Strouhalová, Dana; Laštovičková, Markéta; Bobál, P.; Macejová, D.; Mosná, B.; Brtko, J.; Bobálová, Janette
2018 - English
This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on tributyl/phenyltin derivatives that suggest the combined antitumor effect when they are used with natural nuclear retinoid receptor ligand all-trans retinoic acid (ATRA). Proteomic strategies connected to PDQuest evaluation were applied in this study. Some specific proteins with tumor process association were chosen for more detail study. Our findings indicate that selected triorganotins derivatives resulted to significantly reduced level of studied proteins belonging to metabolic pathway or to other cellular processes as apoptosis or epithelial–mesenchymal transition. In the case of vimentin, the key protein of EMT process, the silencing was set up by triorganotin compounds and significantly enhanced by combination with ATRA. This additive effect on downregulation can result from the potential engagement of RXR/RAR heterodimer, considering co-addition of both partner ligands of mentioned heterodimer to the treatment.
Keywords:
nuclear retinoid X receptors; triorganotin compounds; breast carcinoma cells
Fulltext is available at external website.
A New evaluation of combined antitumor effects of natural and synthetic nuclear retinoid receptor ligands in human breast carcinoma cells
This work represents the study of the effects of biologically active ligands of nuclear retinoid X receptors, triorganotin compounds, on proteomic profile of MDA-MB-231 cells. We focused primarily on ...
Fate of smelter dusts in temperate and semi-arid soils
Ettler, V.; Jarošíková, A.; Mihaljevič, M.; Kříbek, B.; Veselovský, F.; Penížek, V.; Vaněk, A.; Šráček, O.; Matoušek, Tomáš; Mapani, B.; Kamona, F.; Nyambe, I.
2017 - English
Our results indicate that, compared to semi-arid areas (where inorganic contaminants are rather immobile in soils despite their high concentrations), a higher potential risk exists in smelter-affected humid areas, where metal(loid) contaminants can be easily released by extensive weathering of particulates in the soil systems.
Keywords:
soils; smelter dusts
Available in digital repository of the ASCR
Fate of smelter dusts in temperate and semi-arid soils
Our results indicate that, compared to semi-arid areas (where inorganic contaminants are rather immobile in soils despite their high concentrations), a higher potential risk exists in smelter-affected ...
Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Vaněčková, T.; Zítka, J.; Hlaváček, Antonín; Adam, V.; Vaculovičová, M.
2017 - English
Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength under near-infrared excitation (typically 980 nm). In this work, we have examined these unique photophysical properties by fluorescence spectrometry and capillary electrophoresis. UCNPs co-doped with Yb(III) and Er(III) were characterized using laboratory-made fluorescence spectrometer. We have exploited and evaluated two excitation sources and the dependence of the fluorescence of UCNPs on the relative excitation power. Moreover, capillary electrophoresis with laser-induced fluorescence (CE-LIF) detection was for the first time used for characterization of the nanoparticles. It was proved that CE-LIF is a valuable method to be used for investigation of upconversion luminiscence and monitoring of the interactions of UCNPs with other molecules of interest.
Keywords:
capillary electrophoresis; fluorescence spectrometry; upconversion nanoparticles
Fulltext is available at external website.
Characterization of upconversion nanoparticles by fluorescence spectrometry and capillary electrophoresis
Upconversion nanoparticles (UCNPs) are a novel class of luminescent tags for applications in life and material sciences. Unlike traditional fluorophores, UCNPs exhibit emission of shorter wavelength ...
Development of microfluidic device for droplet generation and microparticle encapsulation
Křivánková, Jana; Foret, František
2016 - English
Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is divided into numerous droplets surrounded by a\ncontinuous immiscible fluid phase. Generated droplets serve then as\nvessels/microcapsules for delivering drugs, nutrients as well as for encapsulating of\nbiologically active particles and cells.\nDescribed experimental study deals with the development of a practical dropletbased\nmicrofluidic chip useful for production of monodisperse water/oil emulsion and\nfor encapsulation of thin-metal magnetic microsheets.
Keywords:
microfluidic device; microparticle encapsulation
Available in digital repository of the ASCR
Development of microfluidic device for droplet generation and microparticle encapsulation
Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is ...
Subsidiary reagens for improvement of enzymatic digestion protocol
Laštovičková, Markéta; Bobáľ, P.; Flodrová, Dana; Bobálová, Janette
2016 - English
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.
Keywords:
proteomic identifications; MALDI-TOF MS; enzymatic digestion; peptides
Available in digital repository of the ASCR
Subsidiary reagens for improvement of enzymatic digestion protocol
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
Čapka, Lukáš; Mikuška, Pavel; Večeřa, Zbyněk
2016 - English
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. The system was applied to the determination of\nDCAs in urban aerosols in Brno. The method was verified using a laboratory generated\nstandard aerosol of DCAs. A sampling of aerosol particles on parallel filter was used\nas a reference method.
Keywords:
atmospheric aerosols; dicarboxylic acids; aerosol collector; ion chromatography
Available in digital repository of the ASCR
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. ...
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří; Roth, Michal
2016 - English
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches.
Keywords:
extraction of bioactive proteins; MALDI-TOF/TOF mass spectrometry; Sambucus nigra L. branches; SDS-PAGE; PHWE
Available in digital repository of the ASCR
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of ...
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Večeřová, Kristýna; Mikuška, Pavel; Oravec, Michal; Kozáčiková, Michaela; Pompeiano, Antonio; Coufalík, Pavel; Urban, Otmar
2016 - English
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week exposure to airborne CdONPs of ecologically\nrelevant size and concentration significantly reduced photosynthetic light use efficiency\nin plants as well as contents of photosynthetic pigments and saccharides. In contrary,\namino acid content was increased under CdONPs. These changes were further\nmodulated by previous growth temperature.
Keywords:
Picea abies; terrestrial ecosystems; CdO nanoparticles
Available in digital repository of the ASCR
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week ...
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2016 - English
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment.
Keywords:
capillary electrophoresis; electrospray-ionization mass spectrometric detection; chlorophenols; sulfonamides
Available in digital repository of the ASCR
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic ...
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