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Preparation, physical-chemical properties and utilization of nanoparticles in bioanalysis
Svobodová, Ivona; Hezinová, Věra; Lišková, Marcela; Přikryl, Jan; Maděránková, D.; Klepárník, Karel; Foret, František
2009 - English
The possibilities of application of nanoparticles in bioanalysis were studied. Applications of quantum dots as a fluorescent markers, silver nanoparticles in SERS and conjucation of gold nanoparticles with antibodies were shown.
Keywords:
bioanalysis; fluorescent probes; nanoparticles
Available at various institutes of the ASCR
Preparation, physical-chemical properties and utilization of nanoparticles in bioanalysis
The possibilities of application of nanoparticles in bioanalysis were studied. Applications of quantum dots as a fluorescent markers, silver nanoparticles in SERS and conjucation of gold nanoparticles ...
Potential of quantum dots for single molecule detection in cell membranes and organelles
Přikryl, Jan; Klepárník, Karel; Svobodová, Ivona; Hezinová, Věra; Lišková, Marcela; Preisler, J.; Foret, František
2009 - English
This contribution is devoted to quantum dots usage for cell imaging in fluorescence microscopy. Fluorescence excitation by evanescence wave (caused by total internal reflection) is compared with conventional epifluorescence microscopy. Příspěvek je věnován využití kvantových teček pro značení buněk ve fluorescenční mikroskopii. Zejména je srovnána excitace fluorescence evanescentní vlnou (vznikající při totálním interním odrazu) s konvenčním epifluorescenčním uspořádáním.
Keywords:
quantum dots; luminescence; fluorescence
Available at various institutes of the ASCR
Potential of quantum dots for single molecule detection in cell membranes and organelles
This contribution is devoted to quantum dots usage for cell imaging in fluorescence microscopy. Fluorescence excitation by evanescence wave (caused by total internal reflection) is compared with ...
Extraction and determination of flavonoids in Stevia rebaudiana leaves by pressurized solvent and HPLC
Hohnová, Barbora; Omelková, J.; Vespalcová, M.; Karásek, Pavel
2009 - English
Determination of selected flavonoids in Stevia rebaudiana leaves by HPLC. Comparison of extraction techniques, PFE, soxhlet extraction and ultrasound assisted extraction.
Keywords:
PFE; HPLC; flavonoids
Available at various institutes of the ASCR
Extraction and determination of flavonoids in Stevia rebaudiana leaves by pressurized solvent and HPLC
Determination of selected flavonoids in Stevia rebaudiana leaves by HPLC. Comparison of extraction techniques, PFE, soxhlet extraction and ultrasound assisted extraction.
Characterization of a new 8-hydroxyquinoline modifed resin gel for DGT technique
Gregušová, Michaela; Dočekal, Bohumil
2009 - English
This study deals with a new resin gel based on Spheron-Oxin® ion exchanger with anchored specific groups of 8-hydroxyquinoline for DGT technique. The performance of this new binding phase was tested for determination of Cd, Cu, Ni, Pb and U under laboratory conditions. The new binding phase with a higher selectivity to race metal species than Chelex 100 could potentially provide more information on metals speciation in aquatic systems.
Keywords:
DGT technique; 8-hydroxychinoline; uranium
Available at various institutes of the ASCR
Characterization of a new 8-hydroxyquinoline modifed resin gel for DGT technique
This study deals with a new resin gel based on Spheron-Oxin® ion exchanger with anchored specific groups of 8-hydroxyquinoline for DGT technique. The performance of this new binding phase was tested ...
Chemical vapour generation of silver for atomic absorption spectrometry: .sup.111./sup.Ag radiotracer study of transport efficiency and in-situ trapping on quartz surface
Musil, Stanislav; Kratzer, Jan; Vobecký, Miloslav; Matoušek, Tomáš
2009 - English
The method of chemical vapour generation of silver by means of sodium tetrahydroborate was futher developed in order to minimize transport losses. The possibility of collection of volatile silver species directly in the atomizer is presented. Metoda generování těkavých forem stříbra pomocí reakce s roztokem tetrahydroborátu byla dále vyvinuta za účelem snížení transportních ztrát. Je prezentována možnost provedení kolekce stříbra přímo v křemenném atomizátoru.
Keywords:
chemical vapour generation; transport efficiency; in-situ trapping
Available at various institutes of the ASCR
Chemical vapour generation of silver for atomic absorption spectrometry: .sup.111./sup.Ag radiotracer study of transport efficiency and in-situ trapping on quartz surface
The method of chemical vapour generation of silver by means of sodium tetrahydroborate was futher developed in order to minimize transport losses. The possibility of collection of volatile silver ...
Chemical vapour generation of silver as a method for sample introduction for atomic absorption spectrometry
Musil, Stanislav; Kratzer, Jan; Vobecký, Miloslav; Rychlovský, P.; Matoušek, Tomáš
2008 - English
A method of silver chemical vapour generation as a sample introduction technique for analytical atomic spectrometry is presented. Optimization of reaction and atomization parametrs was carried out and the efficiency of the chemical vapour generation was studied by means of radiotracer experiments. V práci je představena metoda chemického generování těkavých forem stříbra jako metoda vnášení vzorku pro analytickou atomovou spektrometrii. Byla provedena optimalizace reakčních a atomizačních podmínek a byla stanovena účinnost generování těkavých forem stříbra pomocí experimentů s radioaktivními izotopy stříbra.
Keywords:
chemical vapour generattion; silver; analytical spectrometry
Available at various institutes of the ASCR
Chemical vapour generation of silver as a method for sample introduction for atomic absorption spectrometry
A method of silver chemical vapour generation as a sample introduction technique for analytical atomic spectrometry is presented. Optimization of reaction and atomization parametrs was carried out and ...
Thioglycolic acid as on-line pre-reductant for speciation analysis of arsenic by selective hydride generation-cryotrapping-AAS
Musil, Stanislav; Matoušek, Tomáš; Rychlovský, P.
2008 - English
This work represents an improvement of current method of speciation analysis for determination of tri- and pentavalent inorganic, mono-, di-, and trimethylated arsenicals using atomic absorption spectrometry as a detector. Presented study shows that thioglycolic acid is suitable for an on-line pre-reduction which simplifies sample pretreatment and increases sample throughput. Práce představuje zlepšení současné metody speciační analýzy pro stanovení trojmocných a pětimocných sloučenin arsenu za použití detekce atomovou absorpční spektrometrií. Kyselina thioglykolová byla shledána vhodná pro on-line předredukci a ulehčuje tak podstatně proces předúpravy vzorků.
Keywords:
HG-AAS; arsenic; on-line pre-reduction
Available at various institutes of the ASCR
Thioglycolic acid as on-line pre-reductant for speciation analysis of arsenic by selective hydride generation-cryotrapping-AAS
This work represents an improvement of current method of speciation analysis for determination of tri- and pentavalent inorganic, mono-, di-, and trimethylated arsenicals using atomic absorption ...
As and Se hydride trapping at the quartz surface - a step forward to in-situ trapping in quartz tube atomizers for AAS
Kratzer, Jan; Dědina, Jiří; Rychlovský, P.
2007 - English
An optimization study of arsine and selenum hydride preconcentration at the quartz surface is presented. A novel apparatus design consisting of a quartz trap integrated with a quartz multiatomizer is described. Práce popisuje optimalizaci podmínek prekoncentrace hydridů arsenu a selenu na křemenném povrchu. Pro tento účel byl navržen nový design aparatury sestávající z křemenného prekoncentračního zařízení integrovaného s křemenným multiatomizátorem.
Keywords:
HG-AAS; quartz surface; hydride trapping
Available at various institutes of the ASCR
As and Se hydride trapping at the quartz surface - a step forward to in-situ trapping in quartz tube atomizers for AAS
An optimization study of arsine and selenum hydride preconcentration at the quartz surface is presented. A novel apparatus design consisting of a quartz trap integrated with a quartz multiatomizer is ...
Development of L-shaped hydride multiatomizer for atomic absorption spectrometry
Grossová, Olga; Dědina, Jiří
2007 - English
A novel design of hydride multiatomizer was described. The performence of several modifications of the multiatomizer with variety of optical tubes, especially porous quartz and multiple perforated quartz, has been discussed. Představen byl nový typ multiatomizátoru hydridů. Diskutovány byla jeho funkčnost v závislosti na užití různých typů optických trubic, zejména z porézního přemenného skla a z křemenného skla mnohonásobně děrovaného.
Keywords:
HG-AAS; multiatomizer; porous quartz
Available at various institutes of the ASCR
Development of L-shaped hydride multiatomizer for atomic absorption spectrometry
A novel design of hydride multiatomizer was described. The performence of several modifications of the multiatomizer with variety of optical tubes, especially porous quartz and multiple perforated ...
Development of microfluidic analytical system for cell electrolysis
Grym, Jakub; Svobodová, Ivona; Klepárník, Karel; Foret, František
2006 - English
Microfluidic devices are expected to speed up the progress in bioanalysis and separation sciences. Ideally, these Lab-on-chip systems integrate all steps of analysis on a one microdevice; however, before the integration, every single unit operation has to be first developed and tested separately. One of such important unit operations is the cell lysis and the following release of the cellular content. Od mikrofluidických systémů se očekává urychení pokroku v bioanalýze a separačních vědách. V ideálním případě systémy Lab-on-chip integrují všechny kroky analýzy v jednom mikrozařízení; ovšem před integrací musí být každá jednotlivá operace vyvinuta a otestována samostatně. Jednou takovou důležitou operací je buněčná lýza a následné uvolnění buněčného obsahu.
Keywords:
cell electrolysis; microfluidic devices
Available at various institutes of the ASCR
Development of microfluidic analytical system for cell electrolysis
Microfluidic devices are expected to speed up the progress in bioanalysis and separation sciences. Ideally, these Lab-on-chip systems integrate all steps of analysis on a one microdevice; however, ...
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