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The involvement of monocytes and dendritic cells in coelias cisease pathogenesis
Tučková, Ludmila; Palová-Jelínková, Lenka; Rožková, D.; Černá, M.; Pecharová, Barbara; Smythies, L.; Smith, P.; Dvořák, M.; Fruhauf, P.; Tlaskalová, Helena
2006 - English
This work studies the involvement of monocytes and dendritic cells in coelias cisease pathogenesis Práce se zabývá studiem monocytů a dendritických buněk v patogenezi celiakie
Keywords:
monocytes; dendritic cells
Available at various institutes of the ASCR
The involvement of monocytes and dendritic cells in coelias cisease pathogenesis
This work studies the involvement of monocytes and dendritic cells in coelias cisease pathogenesis
Práce se zabývá studiem monocytů a dendritických buněk v patogenezi celiakie
The difference in cytokine response against enteropathogenic or non pathogenic E. coli
Šplíchal, Igor; Trebichavský, Ilja; Šplíchalová, Alla; Kofroňová, Olga
2006 - English
Germ-free pigs were orally infected with 108 CFU of E.coli bacteria for 24 hours - either with a non-pathogenic O86 strain or with an enteropathogenic O55 strain. Both microbes translocated into the gut and mesenteric lymph nodes of gnotobiotic pigs and values of IL-8 and IL-10 correlated with bacterial virulence Bezmikrobní selata byla orálně infikována 108 CFU E.coli po dobu 24 hodin nepatogenním kmenem O86 nebo patogenním kmenem O55. Oba kmeny translokovaly do střeva a mezenteriálních lymfatických uzlin gnotobiotických selat a hodnoty IL-8 a IL-10 korelovaly s bakteriální virulencí
Keywords:
e.coli; germ-free; gnotobiotic
Available at various institutes of the ASCR
The difference in cytokine response against enteropathogenic or non pathogenic E. coli
Germ-free pigs were orally infected with 108 CFU of E.coli bacteria for 24 hours - either with a non-pathogenic O86 strain or with an enteropathogenic O55 strain. Both microbes translocated into the ...
Influence of intestinal bacteria on colitis development in SCID mice reconstituted with CD4+CD45RB high T cells and on changes of tight junctions (TJs) and Paneth cells
Štěpánková, Renata; Powrie, F.; Kofroňová, Olga; Kozáková, Hana; Hudcovic, Tomáš; Hrnčíř, Tomáš; Tlaskalová, Helena
2006 - English
In the model of colitis developing in T cell reconstituted SCID mice pronounced morphological impairment of colon, was accompanied by structural changes of tight junctions and decreased expression of alpha defensins in terminal ileum Na modelu kolitidy, která se vyvolá u SCID myší po přenosu T buněk CD4+CD45RBhigh jsme zjistili poškození tight junction enterocytů v terminálním ileu a snížení produkce alpha defensinů
Keywords:
colitis; t cells
Available at various institutes of the ASCR
Influence of intestinal bacteria on colitis development in SCID mice reconstituted with CD4+CD45RB high T cells and on changes of tight junctions (TJs) and Paneth cells
In the model of colitis developing in T cell reconstituted SCID mice pronounced morphological impairment of colon, was accompanied by structural changes of tight junctions and decreased expression of ...
Susceptibility to nasal or oral tolerance induction is not dependent on the presence of microflora
Repa, A.; Kozáková, Hana; Hudcovic, Tomáš; Štěpánková, Renata; Hrnčíř, Tomáš; Tlaskalová, Helena; Pollak, A.; Wiedermann, U.
2006 - English
The development of mucosal tolerance to major allergic component of birch pollen in relationship with presence of intestinal microflora was the aim of presented study. Germ-free (GF) or conventionally reared (CV) BALB/c mice were intragastrically or intranasally pre-treated with Bet v 1 prior to sensitization performed by subcutaneous injections of Bet v 1. Oral as well as intranasal tolerance induction led to a significant reduction of allergen-specific antibody levels and IgE basophil degranulation, as well as cytokine production in vitro in both GF and CV mice. The importance of the microflora for mucosal tolerance induction in GF mice remains therefore a matter of debate Vliv střevní mikroflóry na navození slizniční tolerance k pylu břízy byl studován na myším modelu. Bezmikrobní a konvenční myši kmene BALB/c byly sondovány Bet v 1 intragastricky a intranasálně a po té byly senzitizovány subkutálně Bet v 1. Byla navozena tolerance nezávisle na přítomnosti bakterií, která se odrazila ve snížené hladině IgE a změněné cytokinové produkci. Důležitost přítomnosti střevní mikrofóry při navození slizniční tolerance u bezmikrobních myších je stále předmětem debaty
Keywords:
allergology; immunology
Available at various institutes of the ASCR
Susceptibility to nasal or oral tolerance induction is not dependent on the presence of microflora
The development of mucosal tolerance to major allergic component of birch pollen in relationship with presence of intestinal microflora was the aim of presented study. Germ-free (GF) or ...
Ochranný účinek E. coli kmen Nissle 1917 a E. coli kmen O83 na vývoj střevního zánětu vyvolaného dextransulfátem sodným u myší
Hudcovic, Tomáš; Štěpánková, Renata; Hrnčíř, Tomáš; Kozáková, Hana; Schwarzer, Martin; Tlaskalová, Helena
2006 - Czech
Tato práce byla zaměřena na zhodnocení probiotických bakterií E. coli strain Nissle 1917 a E. coli strain O83 .Vývoj střevního zánětu byl studován v DSS modelu ulcerózní kolitidy. SCID myším byly aplikovány probiotické bakterie jak intragastricky, tak intrarektálně. Při intragastrické aplikaci se u experimentálních zvířat objevily těžké zánětlivé změny na sliznici tlustého střeva – část descendens a produkce prozánětlivých cytokinů TNF – alpha a IL – 6, které byly vyizolovány z fragmentových kultur tlustého střeva – části colon – descendens, byla výrazně zvýšena proti skupině u které byly bakterie aplikovány intrarektálně a kde se projevil protektivní vliv aplikovaných bakterií The study was focused on the evaluation of probiotic bacteria E. coli strain Nissle 1917 and E. coli strain O83 and their role in the development of intestinal inflammation in DSS model of ulcerative colitis and the route of the application. Intragastrically tubing SCID mice ( with severe combined immunodeficiency) had heavy chages (inflammation) of the colon – part descendens. Intrarectally application had a protective effect on the development of intestinal inflammation – part colon descendens. Proinflammatory cytokines TNF – alpha and IL – 6 were reduce markedly in fragment culture from colon - descendens
Keywords:
ulcerative colitis; dextran sulfate sodium model
Available at various institutes of the ASCR
Ochranný účinek E. coli kmen Nissle 1917 a E. coli kmen O83 na vývoj střevního zánětu vyvolaného dextransulfátem sodným u myší
Tato práce byla zaměřena na zhodnocení probiotických bakterií E. coli strain Nissle 1917 a E. coli strain O83 .Vývoj střevního zánětu byl studován v DSS modelu ulcerózní kolitidy. SCID myším byly ...
Untranslated RNA of Corynebacterium glutamicum induced by heat shock
Zemanová, Martina; Pátek, Miroslav; Nešvera, Jan
2006 - English
The first case of chromosomally encoded, untranslated, regulatory RNA in the amino acid-producing bacterium Corynebacterium glutamicum is described. The possible role of this novel regulatory RNA of C. glutamicum in control of cell response to external stress factors is in agreement with observed induction of various small untranslated RNAs in Escherichia coli by a variety of environmental stress conditions Je popsán první případ chromosomálně kódované, nepřekládané, regulační RNA u aminokyseliny produkující bakterie Corynebacterium glutamicum. Pravděpodobná funkce této nové regulační RNA u C. glutamicum spočívá v regulaci buněčné odpovědi vůči vnějším stresovým faktorům, což je v souladu s již pozorovanými případy indukce různých malých nepřekládaných RNA u Escherichia coli v závislosti na různých stresových podmínkách
Keywords:
corynebacterium glutamicum; rna
Available at various institutes of the ASCR
Untranslated RNA of Corynebacterium glutamicum induced by heat shock
The first case of chromosomally encoded, untranslated, regulatory RNA in the amino acid-producing bacterium Corynebacterium glutamicum is described. The possible role of this novel regulatory RNA of ...
Ammonia signalling in Saccharomyces cerevisiae colonies: Expression of amino acids transporters in SPS sensor defective strains
Harant, Karel; Váchová, Libuše; Palková, Z.
2006 - English
The capability of yeast colonies to produce ammonia depends on correct function of SPS sensor composed of Ssy1p, Ssy5p and Ptr3p. The obtained data indicate that SPS sensor influences the expression of amino acid transporter genes in yeast colonies by different way when compared with its function in yeast liquid cultures Schopnost kvasinkových kolonií produkovat amoniak je závislá na správné funkci sensoru SPS, který se skládá z Ssy1p, Ssy5p a Ptr3p. Získaná data naznačují, že SPS sensor ovlivňuje expresi genů transportérů aminokyselin v kvasinkových koloniích jiným způsobem, než je tomu v tekutých kulturách kvasinek
Keywords:
saccharomyces cerevisae; ammonia
Available at various institutes of the ASCR
Ammonia signalling in Saccharomyces cerevisiae colonies: Expression of amino acids transporters in SPS sensor defective strains
The capability of yeast colonies to produce ammonia depends on correct function of SPS sensor composed of Ssy1p, Ssy5p and Ptr3p. The obtained data indicate that SPS sensor influences the expression ...
Escherichia coli O83:K24:H31 – a potential live vaccine delivery vector
Šimšová, Marcela; Schwarzer, Martin; Hrnčíř, Tomáš; Hudcovic, Tomáš; Kozáková, Hana; Šebo, Peter
2006 - English
The aim of our study was to create a mucosal vaccine for impoverished population. We used Escherichia coli O83:K24:H31 as live vector. It produced fused antigen M. tuberculosis fused antigens Ag85B-Esat6 (H1) which was inserted into a surface-exposed loop of the outer membrane protein LamB. Mice were given the bacteria according to the immunization protocol, they received free soluble H1 antigen with the mucosal adjuvant LTK63 as intranasal boost. We observed increase of total IgA on the intestine mucosa after colonization of E. coli O83 and induction of specific anti-H1 IgA level in small intestine Cílem naší práce bylo vytvořit mukosální vakcínu pro třetí svět. Jako vektor jsme použili Escherichia coli O83:K24:H31. Ta byla geneticky manipulována k produkci fůzního antigenu Ag85B-Esat6 (H1) M. tuberculosis, který byl vložen do proteinu LamB. Myši dostaly baktérii podle imunizačního protokolu a byly intranasálně boostovány LTK63 a volným H1 proteinem. Pozorovali jsme zvýšení celkového IgA ve střevě po osazení E. coli O83 a indukci specifických anti-H1 protilátek v tenkém střevě
Keywords:
mucosal vaccine; e coli; immunization
Available at various institutes of the ASCR
Escherichia coli O83:K24:H31 – a potential live vaccine delivery vector
The aim of our study was to create a mucosal vaccine for impoverished population. We used Escherichia coli O83:K24:H31 as live vector. It produced fused antigen M. tuberculosis fused antigens ...
Structure and function of tmRNA controlling translation in Streptomycetes
Bobek, Jan; Palečková, Petra; Mikulík, Karel
2006 - English
The functional trans-translation system is important to allow survival of antibiotic producing microorganism under unfavourable conditions. The ssrA genes from S. coelicolor, S. collinus, S. aureofaciens and S. griseus were amplified and subjected to sequence analysis. To investigate the role of tmRNA in streptomycetes we have examined the level of tmRNA during the growth and differentiation under various physiological conditions Funkční transtranslační systém pro přežití bakterie v nepříznivých podmínkách. Geny ssrA ze čtyř druhů streptomycet byly amplifikovány a podrobeny sekvenční analýze. Byla studována hladina tmRNA v průběhu růstu a diferenciace za různých fyziologických podmínek
Keywords:
streptomycetes; tmrna
Available at various institutes of the ASCR
Structure and function of tmRNA controlling translation in Streptomycetes
The functional trans-translation system is important to allow survival of antibiotic producing microorganism under unfavourable conditions. The ssrA genes from S. coelicolor, S. collinus, S. ...
Partial deletion of the RPS0A-binding domain of TIF32/eIF3A affects 40S ribosome-association of the multifactor complex and severely impairs reinitiation on the GCN4 mRNA leader
Szamecz, Bela; Rutkai, Edit; Nielsen, K. H.; Valášek, Leoš
2006 - English
Yeast Initiation factor 3 (eIF3) occurs together with the eIF2.GTP.Met-tRNAiMet ternary complex (TC) and eIFs 1 and 5 in a pre-formed unit designated the multifactor complex (MFC) that was implicated in playing a critical role in ecient recruitment of TC and mRNA to the 40S ribosomes and stimulation of the post-assembly processes such as scanning and AUG recognition. In ecort to identify binding sites of the MFC on the 40S ribosome, we previously demonstrated that deletion of the rst 199 amino acids of the N-terminal domain (NTD) of the TIF32 subunit of eIF3/eIF3a, in the presence of a wild-type gene, completely eliminated association of the mutant MFC with the 40S ribosome without aecting its overall integrity. In addition, we showed that the TIF32-NTD contains a binding site for the C-terminal domain (CTD) of the small ribosomal protein 0A (RPS0A) that is located on the solvent side of the 40S subunit where the main body of eIF3 was proposed to reside Práce pojednáva o studiu inciace translace a specifické úloze translačního iniciačního faktoru eIF3
Keywords:
translation; gcn4
Available at various institutes of the ASCR
Partial deletion of the RPS0A-binding domain of TIF32/eIF3A affects 40S ribosome-association of the multifactor complex and severely impairs reinitiation on the GCN4 mRNA leader
Yeast Initiation factor 3 (eIF3) occurs together with the eIF2.GTP.Met-tRNAiMet ternary complex (TC) and eIFs 1 and 5 in a pre-formed unit designated the multifactor complex (MFC) that was implicated ...
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