No (3368)
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3368
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Forest Soil Physico-Chemical Sorption Spatial Links in Central-European Systems of Site Geographical Divisions
Samec, Pavel; Balková, M.; Kučera, A.
2020 - English
Spatial links among soil property values influence variability of soil sorption. The aim of this study was to compare the effect of geotectonic, soil-and biogeographical divisions on forest soil physicochemical sorption spatial links. The effect was investigated through optimal model selection and its comparison among the soil cover division systems of Central-European Highlands in the Czech Republic. Optimal model was selected through the closest-fitting estimations of linear global and local regressions between sorption and chemical or geomorphological forest soil properties. The optimal model comparison was carried out through linear regression with bedrock type proportion (geodiversity) at units of particular division systems. Chemical properties influenced forest soil sorption more than relief. Soil base saturation (BS) was locally more divided than cation exchange capacity (CEC). Local regression of BS with Al2O3 was divided by geotectonic systems the most, but it was not influenced by geodiversity. Geodiversity influenced local regression of CEC with Al2O3 in soil regions as well as regression with C/N in biogeographical regions. Differences between spatial links of BS and CEC suggest that forest soil sorption is divided into multi-level clusters. Spatial link modelling of soil sorption can optimalize forest growth condition division for effective management use.
Keywords:
cation-exchange capacity; organic-matter; weighted regression; resolution; fractions; ratios; soil aluminium; base saturation; geodiversity; geographically weighted regression
Available at various institutes of the ASCR
Forest Soil Physico-Chemical Sorption Spatial Links in Central-European Systems of Site Geographical Divisions
Spatial links among soil property values influence variability of soil sorption. The aim of this study was to compare the effect of geotectonic, soil-and biogeographical divisions on forest soil ...
EDWork Manual IV. User Guide to EDWork 4.1
Sobolík, Václav; Wein, Ondřej
2020 - English
The presen report would serve as a user guide to the software package EDWork 4.1.
Keywords:
electrodiffusion technique; edwork manual; user guide
Available at various institutes of the ASCR
EDWork Manual IV. User Guide to EDWork 4.1
The presen report would serve as a user guide to the software package EDWork 4.1.
Determination of heavy metal poisoning antidote 2,3-dimercapto-1-propanesulfonic acid using silver solid amalgam electrode
Choińska-Mlynarczyk, Marta; Hrdlička, Vojtěch; Redondo, B. R.; Barek, J.; Navrátil, Tomáš
2020 - English
2,3-Dimercapto-1-propane-sulfonic acid (DMPS) was investigated using direct current voltammetry (DCV), differential pulse cathodic stripping voltammetry (DPCSV), differential pulse anodic stripping voltammetry (DPASV), and elimination voltammetry with linear scan (EVLS) at a polished (p-AgSAE)and at a meniscus modified silver solid amalgam electrode (m-AgSAE). EVLS confirmed two consecutive reductions with coupled proton/electron transfer. Voltammetric titrations of DMPS with Pb2+ proved complex formation, with limits of quantification (LOQs) and detection (LODs) 0.3 and 0.1 mu mol L-1 at m-AgSAE and 0.8 and 0.3 mu mol L-1 at p-AgSAE, respectively. Determination of DMPS in commercial drug Dimaval and human urine samples confirmed practical applicability of the developed method.
Keywords:
cathodic stripping voltammetry; 2,3-dimercapto-1-pro-panesulfonic acid; elimination voltammetry with linear scan
Available on request at various institutes of the ASCR
Determination of heavy metal poisoning antidote 2,3-dimercapto-1-propanesulfonic acid using silver solid amalgam electrode
2,3-Dimercapto-1-propane-sulfonic acid (DMPS) was investigated using direct current voltammetry (DCV), differential pulse cathodic stripping voltammetry (DPCSV), differential pulse anodic stripping ...
Career in Polymers XI, Book of Abstracts
Kobera, Libor
2019 - English
The book of abstracts contains the summaries of all the contributions to the Career in Polymers XI., workshop organized by the Institute of Macromolecular Chemistry AS CR, in Prague, 28-29 June, 2019. This meeting was organized under the auspices of UNESCO/IUPAC course in Polymer Science, and supported by Strategy AV21.
Keywords:
polymer science; career in polymers; UNESCO course in Polymer Science
Available at various institutes of the ASCR
Career in Polymers XI, Book of Abstracts
The book of abstracts contains the summaries of all the contributions to the Career in Polymers XI., workshop organized by the Institute of Macromolecular Chemistry AS CR, in Prague, 28-29 June, 2019. ...
Effect of metal sensitizers on photochemical vapor generation of bismuth for analytical atomic spectrometry
Vyhnanovský, Jaromír; Yildiz, D.; Musil, Stanislav
2019 - English
Three new sensitizers with a significant positive effect on the photochemical vapor generation of bismuth were described. Cobalt was found to be the most effective and led to a significant enhancement of the signal.
Keywords:
photochemical volatile species generation; bismuth; AAS
Available at various institutes of the ASCR
Effect of metal sensitizers on photochemical vapor generation of bismuth for analytical atomic spectrometry
Three new sensitizers with a significant positive effect on the photochemical vapor generation of bismuth were described. Cobalt was found to be the most effective and led to a significant enhancement ...
Souhrnná výzkumná zpráva Aalto 1903
Hanuš, Jan; Fabiánek, Tomáš; Fajmon, Lukáš
2019 - English
Within the airborne hyperspectral campaign for a Finnish university Aalto University were scanned two locations - Hyytiälä (FI) - Järvselja (EST). The scanned processed data is used for other scientific purposes.\nAbstrakt: V rámci letecké hyperspektrální kampaňe pro Finskou univerzitu Aalto-yliopisto byly nasnímány dvě lokality - Hyytiälä (FI) - Järvselja (EST). Předaná zpracovaná data jsou používány pro další vědecké účely.
Keywords:
flis; hyperspectral; data; lidar; processing
Available at various institutes of the ASCR
Souhrnná výzkumná zpráva Aalto 1903
Within the airborne hyperspectral campaign for a Finnish university Aalto University were scanned two locations - Hyytiälä (FI) - Järvselja (EST). The scanned processed data is used for other ...
Adhesion and growth of adipose tissue-derived stem cells on fibrin assemblies with attached growth factors for tissue engineering of heart valves
Filová, Elena; Trávníčková, Martina; Knitlová, Jarmila; Matějka, Roman; Kučerová, Johanka; Riedelová, Zuzana; Brynda, Eduard; Bačáková, Lucie
2019 - English
Currently used xenogeneic biological heart valve prostheses are decellularized and crosslinked with glutaraldehyde. These grafts usually undergo degeneration and calcification. Pericardium-based heart valve prostheses, re-seeded with autologous cells, i.e. Adipose tissue-derived cells (ASCs) and endothelial cells, could have longer durability and biocompatibility. In order to improve the adhesion of cells and their ingrowth into decellularized pericardium, various fibrin (Fb) layers were developed, i.e. Fb, Fb with covalently bound heparin (H), Fb with either vascular endothelial growth factor (VEGF) or fibroblast growth factor 2 (FGF) in various concentrations (1 ng/ml, 10 ng/ml, 100 ng/ml) or with both VEGF and FGF (100 ng/ml). Growth factors were attached onto Fb via heparin or were adsorbed. ASCs were seeded on theses layers in a DMEM medium supplemented with 2 % of fetal bovine serum, TGFβ1 and BMP-4 (both 2.5 ng/ml), and with ascorbic acid. Cell adhesion and growth/viability was assessed by counted cell number/MTS evaluation. ASCs were stained for differentiation markers of smooth muscle cells, such as alpha-actin, calponin, and myosin heavy chain. On day 7, ASCs on Fb-H-VEGF layers produced both calponin and alpha-actin. An increased FGF concentration caused reduced calponin staining of ASCs. Lack of heparin in fibrin assemblies with growth factors inhibited the production of both alpha-actin and calponin in ASCs. The highest ASCs density/viability was found on Fb-H-VEGF-FGF layer. The proper formulation of fibrin coatings could be favorable for ASCs growth and differentiation and could subsequently support endothelialization of cardiovascular prostheses with endothelial cells.
Keywords:
adipose tissue-derived stem cells; fibrin assemblies; growth factors; heart valves
Available at various institutes of the ASCR
Adhesion and growth of adipose tissue-derived stem cells on fibrin assemblies with attached growth factors for tissue engineering of heart valves
Currently used xenogeneic biological heart valve prostheses are decellularized and crosslinked with glutaraldehyde. These grafts usually undergo degeneration and calcification. Pericardium-based heart ...
Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.
Sushytskyi, L.; Lukáč, Pavol; Bleha, R.; Čopíková, J.; Vannucci, Luca; Sinica, A.; Kaštánek, P.
2019 - English
Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW (>1000 kDa and 98,3 kDa) polysaccharides (CPS1 and CPS3) were eluted with water and 0.5M NaCl on DEAE column, and heteropolysaccharide with MW of 15 kDa (CPS2) was also eluted with water on DEAE. The former fractions were identified as branched rhamnans, the latter one as branched xylogalactofuranan of unusual structure. CPS1 shown properties of unstable polimer: according to GPC analysis and by using light scattering and RI detectors it was found, that some fraction of polymers (260-800 kDa) have self-assembling properties in aqueous solution, and MW of this fraction decreased during some time while polimer with initially bigger MW (>1000 kDa) became bigger. CPS3 was eluted with 0.3-0.5 M of NaCl on DEAE Sepharose FF column and have MW of 98,3 kDa. Cell toxicity of the products were tested in vitro on cell cultures for different concentrations
Keywords:
Mutant alga; Chlorella vulgaris G11; chromatography; polymer; cell cultures
Available at various institutes of the ASCR
Purification of water soluble bioactive polysaccharides from biomass of heterotrophic mutant alga Chlorella vulgaris G11.
Water extract obtained from biomass of mutant alga Chlorella vulgaris G11 was deproteinized and then purified with AEC on DEAE Sepharose FF and SEC on Sepaphadex G-75 superfine columns. Two high MW ...
Metals in atmospheric particulate matter
Barboříková, H.; Mikuška, Pavel; Hegrová, J.; Pokorná, Petra; Krajčovič, J.
2019 - English
Atmospheric pollution is one of the most serious global enviroment problem. Metals constitute important class of pollutants which have received athe attention of researchers all over the world. Metals have strong potential to be adsorbed on particulate matter and than enter the human body through inhalation causing several health issue (asthma, lung cancer).
Keywords:
aerosol; metals
Available at various institutes of the ASCR
Metals in atmospheric particulate matter
Atmospheric pollution is one of the most serious global enviroment problem. Metals constitute important class of pollutants which have received athe attention of researchers all over the world. Metals ...
Structural characterization of fatty acids with triple bond and unusual double bond positions by HPLC/APCI-MS2
Horká, Petra; Vrkoslav, Vladimír; Cvačka, Josef
2019 - English
Fatty acid methyl esters (FAMEs) were analyzed by reversed-phase HPLC coupled to atmospheric pressure chemical ionization (APCI) mass spectrometry. Acetonitrile was used as mobile phase. The gas-phase reactions of acetonitrile and unsaturated FAMEs in the APCI source provided [M + C3H5N]+• adducts. Subsequent MS/MS analysis of these ions gave diagnostic fragments. The formation and fragmentation of the acetonitrile-related adducts were utilized for the structural characterization of FAME with isolated double bonds in (Z)-9,19-hexacosadienoic acid methyl ester, FAME with conjugated double bonds in (Z)-9,11-octadecadienoic acid methyl ester and FAME with a triple bond in 9-octadecynoic acid methyl ester.
Keywords:
acetonitrile-related adducts; acetylenic lipids; multiple bond localization; mass spectrometry
Available at various institutes of the ASCR
Structural characterization of fatty acids with triple bond and unusual double bond positions by HPLC/APCI-MS2
Fatty acid methyl esters (FAMEs) were analyzed by reversed-phase HPLC coupled to atmospheric pressure chemical ionization (APCI) mass spectrometry. Acetonitrile was used as mobile phase. The gas-phase ...
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