No (286)
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Using of divergent flow isoelectric focusing and capillary liquid
Duša, Filip; Moravcová, Dana; Kahle, Vladislav; Šlais, Karel
2011 - Czech
Separation of analytes from complex matrices play important role in their further identification. In this article we propose two-dimensional separation method of bovine serum albumin tryptic peptides. At the first dimension peptides are separated by divergent flow isoelectric focusing followed by reverse-phase liquid chromatography as the second dimension. Due to composition of input mixture it is possible to use autofocusing mode in first dimension. In this mode peptides are focused without addition of carrier ampholytes. Chromatograms from the second dimension were processed into contour map. Acquired data showed good separation efficiency of isoelectric focusing. Autofocusing mode enabled better resolution of peptides with close isoelectric points. Separace analytů z komplexních směsí dnes hraje významnou roli při jejich další identifikaci. V článku je navržena metoda dvoudimenzionální separace směsi tryptických peptidů hovězího sérového albuminu. V první dimenzi jsou peptidy rozděleny isoelektrickou fokusací v rozbíhavém toku a v druhé dimenzi následuje kapalinová chromatografie s reverzní fází. Díky charakteru vstupní směsi je možné u izoelektrické fokusace využít autofokusaci peptidů, tzn. fokusaci bez přídavku nosných amfolytů. Chromatogramy z druhé dimenze byly zpracovány do podoby vrstevnicové mapy. Získaná data ukázala dobrou separační účinnost izoelektrické fokusace a díky autofokusaci bylo možné rozdělit i peptidy s blízkými izoelektrickými body.
Keywords:
peptides; DF-IEF; RPLC
Available at various institutes of the ASCR
Using of divergent flow isoelectric focusing and capillary liquid
Separation of analytes from complex matrices play important role in their further identification. In this article we propose two-dimensional separation method of bovine serum albumin tryptic ...
Determination of organic compounds in PM1 in Brno and Šlapanice
Křůmal, Kamil; Mikuška, Pavel; Vojtěšek, Martin; Večeřa, Zbyněk
2011 - Czech
Monosaccharide anhydrides, polyaromatic hydrocarbons, hopanes, steranes and monocarboxylic acids were analysed in PM1. Contribution deals with extractions of these compounds from filters, preparations of samples, analyses by GC/MS and discussions of results of analysis. V PM1 aerosolu byly analyzovány anhydridy monosacharidů, polyaromatické uhlovodíky, hopany, sterany a monokarboxylové kyseliny. Příspěvek se zabývá extrakcemi těchto sloučenin z filtrů, přípravami vzorků, analýzami metodou GC/MS a diskusemi výsledků analýz.
Keywords:
organic compounds; PM1
Available at various institutes of the ASCR
Determination of organic compounds in PM1 in Brno and Šlapanice
Monosaccharide anhydrides, polyaromatic hydrocarbons, hopanes, steranes and monocarboxylic acids were analysed in PM1. Contribution deals with extractions of these compounds from filters, preparations ...
Analysis of saccharides and markers of wood combustion in atmospheric aerosols
Kubátková, Nela; Křůmal, Kamil; Mikuška, Pavel; Večeřa, Zbyněk
2011 - Czech
Present study deals with the analysis of saccharides (monosaccharides, disaccharides and alditols), monosaccharide anhydrides and markers of biomass and wood combustion in atmospheric aerosol and comparison of their concentrations in term of seasons. Studie se zabývá analýzou sacharidů (monosacharidy, disacharidy a alditoly), anhydridů monosacharidů a markerů spalování biomasy a dřeva v atmosférickém aerosolu a porovnáním jejich koncentrací v průběhu jednotlivých ročních období.
Keywords:
atmospheric aerosol PM2,5; saccharides; markers of biomass and wood combustion
Available at various institutes of the ASCR
Analysis of saccharides and markers of wood combustion in atmospheric aerosols
Present study deals with the analysis of saccharides (monosaccharides, disaccharides and alditols), monosaccharide anhydrides and markers of biomass and wood combustion in atmospheric aerosol and ...
Unique exposure system for the whole body inhalation experiments with small animals
Večeřa, Zbyněk; Mikuška, Pavel; Moravec, Pavel; Smolík, Jiří
2011 - English
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of inhalated nano-sized particles (Pb, Cd) and their allocation in organs“. The inhalation chamber builds from a glass and stainless steel and makes possible long-term inhalation procedures (up to 60 days, 24 hours/day) with up to four discrete groups of mice population (up to 60 individuals each) or of rats population (up to 20 individuals each) under controlled illumination, temperature, relative humidity, doses (0.5 x 106 - 5 x 106 particles/cm3) and size (10-60 nm) of nano-sized particles which are synthesized via aerosol route in a hot wall tube flow reactor using metal organic precursors.
Keywords:
inhalation chamber; chronic exposure studies of small animals; long-term inhalation procedures
Available at various institutes of the ASCR
Unique exposure system for the whole body inhalation experiments with small animals
The especially inhalation chamber is constructed for chronic exposure studies of small animals to nanoparticles under strictly controlled conditions in frame of project „Study of transport of ...
Seasonal variation in chemical composition of submicron urban aerosol in Brno
Mikuška, Pavel; Křůmal, Kamil; Večeřa, Zbyněk; Vojtěšek, Martin
2011 - English
Submicron aerosol particles in the size fraction PM1 collected in Brno in winter and summer of 2009 were analysed for both selected inorganic (ions, metals) and organic components (PAHs, hopanes and steranes, monosaccharide anhydrides, monocarboxylic acids). Special attention was paid to analysis of organic molecular markers to identify the major emission sources of aerosols.
Keywords:
submicron aerosol; chemical composition; emission sources
Available at various institutes of the ASCR
Seasonal variation in chemical composition of submicron urban aerosol in Brno
Submicron aerosol particles in the size fraction PM1 collected in Brno in winter and summer of 2009 were analysed for both selected inorganic (ions, metals) and organic components (PAHs, hopanes and ...
Comparison of concentrations of organic compounds in PM1 aerosol in Brno and Šlapanice
Křůmal, Kamil; Mikuška, Pavel; Večeřa, Zbyněk
2011 - Czech
In this work the organic compounds and organic markers used for the identification of sources of aerosols were monitored. Monosaccharide anhydrides are emitted from biomass combustion, polyaromatic hydrocarbons from traffic and incomplete combustion, hopanes from traffic and coal combustion, steranes from traffic and fatty acids from cooking. V této práci byly sledovány koncentrace organických sloučenin a markerů, které slouží pro identifikaci zdrojů atmosférických aerosolů. Anhydridy monosacharidů pocházejí ze spalování biomasy, polyaromatické uhlovodíky z dopravy a nedokonalého spalování, hopany z dopravy a spalování uhlí, sterany z dopravy a mastné kyseliny z vaření.
Keywords:
monosaccharide anhydrides; hopanes; PAHs
Available at various institutes of the ASCR
Comparison of concentrations of organic compounds in PM1 aerosol in Brno and Šlapanice
In this work the organic compounds and organic markers used for the identification of sources of aerosols were monitored. Monosaccharide anhydrides are emitted from biomass combustion, polyaromatic ...
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Lišková, Marcela; Voráčová, Ivona; Hezinová, Věra; Klepárník, Karel; Foret, František
2011 - English
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We linked QD with annexin V, anti-CD3 and anti-PCNA. We labeled T-lymphocytes, apoptotic lymphocyte, mouse duodenum tissue and tumor tissue.
Keywords:
quantum dots; cell labeling; conjugation
Available at various institutes of the ASCR
Immunofluorescent labeling of tissues and free cells via QD-protein conjugates
Preparation of quantum dots and using their QD-protein conjugates in immunofluorescent labeling of cells and tissues. For conjugation was used method viaEDC/sulfo-NHS zero-length cross-linkers. We ...
Semi-continuous analysis of water-soluble fraction of metals in atmospheric aerosols
Vojtěšek, Martin; Mikuška, Pavel; Večeřa, Zbyněk; Křůmal, Kamil
2011 - Czech
Characterization of new method for the semi-continuous determination of trace concentrations of water-soluble metalls in atmospheric aerosols with time resolution of 30 min. Fe3+, Co2+ and Cu2+ were selected as model metals. Charakterizace nové metody pro semi-kontinuální stanovení stopových koncentrací ve vodě rozpustné frakce kovů v atmosférickém aerosolu s časovým rozlišením 30 min. Modelovými kovy byly zvoleny Fe3+, Co2+ a Cu2+.
Keywords:
metals; semi-continuous system; atmospheric aerosols
Available at various institutes of the ASCR
Semi-continuous analysis of water-soluble fraction of metals in atmospheric aerosols
Characterization of new method for the semi-continuous determination of trace concentrations of water-soluble metalls in atmospheric aerosols with time resolution of 30 min. Fe3+, Co2+ and Cu2+ were ...
Luminescence of nanoparticles - quantum dots
Voráčová, Ivona
2011 - Czech
Compilation of materials, physical-chemical properties, synthesis and applications of quantum dots. Shrnutí materiálů, fyzikálně-chemických vlastností, přípravy a aplikací kvantových teček.
Keywords:
quantum dots; nanoparticles; luminescence
Available at various institutes of the ASCR
Luminescence of nanoparticles - quantum dots
Compilation of materials, physical-chemical properties, synthesis and applications of quantum dots.
Analysis of hopanes and steranes in PM1 in Brno and Šlapanice
Křůmal, Kamil; Mikuška, Pavel; Vojtěšek, Martin; Večeřa, Zbyněk
2011 - Czech
Contribution deals with analysis of hopanes and steranes in Brno and Šlapanice. Steranes are markers for traffic and hopanes for traffic and coal combustion. Příspěvek se zabývá analýzou hopanů a steranů v Brně a Šlapanicích. Sterany se řadí mezi markery pro dopravu a hopany mezi markery pro dopravu a spalování uhlí.
Keywords:
hopanes; steranes; PM1
Available at various institutes of the ASCR
Analysis of hopanes and steranes in PM1 in Brno and Šlapanice
Contribution deals with analysis of hopanes and steranes in Brno and Šlapanice. Steranes are markers for traffic and hopanes for traffic and coal combustion.
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