Number of found documents: 51
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Polymeric nanofibrous scaffolds reinforced with diamond and ceramic nanoparticles for bone tissue engineering
Bačáková, Lucie; Pařízek, Martin; Staňková, Ľubica; Novotná, Katarína; Douglas, T.E.L.; Brady, M. A.; Kromka, Alexander; Potocký, Štěpán; Stránská, D.
2015 - English
Three types of nanofibrous scaffolds were prepared by electrospining: (1) poly(lactide-co-glycoside) (PLGA) scaffolds reinforced with 23 wt.% of diamond nanoparticles (DNPs), (2) poly(L-lactide) (PLLA) scaffolds with DNPs in concentration ranging from from 0.4 wt.% to 12.3 wt.%, and (3) PLLA scaffolds with 5 wt.% or 15 wt.% of hydroxyapatite (HAp) nanoparticles. The diameter of the nanofibers ranged between 160 and 729 nm. The nanofibers with nanoparticles were thicker and the void spaces among them were smaller. Mechanical properties of the nanoparticle-loaded scaffolds were better, as demonstrated by a rupture test in scaffolds with DNPs and by a creep behavior test in scaffolds with HAp. On PLGA scaffolds with DNPs, the human osteoblast-like MG-63 cells adhered in similar numbers and grew with similar kinetics as on pure PLGA scaffolds. Human bone marrow mesenchymal stem cells grew faster and reached higher population densities on PLGA-DNP scaffolds. However, on PLLA-based scaffolds, the activity of mitochondrial enzymes and concentration of osteocalcin in MG-63 cells decreased with increasing DNP concentration. On the other hand, the metabolic activity of MG-63 cells and content of osteocalcin in these cells were positively correlated with the HAp concentration in PLLA scaffolds. Thus, PLGA nanofibers with 23 wt% of DNPs and PLLA nanofibers with 5 and particularly 15 wt.% of HAp seem to be promising for bone tissue engineering. Keywords: material nanostructure; nanofibers; osteogenic cells; bone implants; regenerative medicine Available at various institutes of the ASCR
Polymeric nanofibrous scaffolds reinforced with diamond and ceramic nanoparticles for bone tissue engineering

Three types of nanofibrous scaffolds were prepared by electrospining: (1) poly(lactide-co-glycoside) (PLGA) scaffolds reinforced with 23 wt.% of diamond nanoparticles (DNPs), (2) poly(L-lactide) ...

Bačáková, Lucie; Pařízek, Martin; Staňková, Ľubica; Novotná, Katarína; Douglas, T.E.L.; Brady, M. A.; Kromka, Alexander; Potocký, Štěpán; Stránská, D.
Fyziologický ústav, 2015

Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina; Bačáková, Lucie; Pařízek, Martin; Vandrovcová, Marta; Musílková, Jana; Lisá, Věra; Gabor, R.; Marvan, J.
2015 - English
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants. Keywords: Ti6-Al4-V; SAOS-2 cells; anodization; bone implants; collagen I; mineralization Available at various institutes of the ASCR
Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization

Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is ...

Doubková, Martina; Bačáková, Lucie; Pařízek, Martin; Vandrovcová, Marta; Musílková, Jana; Lisá, Věra; Gabor, R.; Marvan, J.
Fyziologický ústav, 2015

Case study of intracranial EEG records of patients with focal cortical dysplasia type I and II
Balach, J.; Ježdík, P.; Čmejla, R.; Kršek, P.; Jiruška, Přemysl
2015 - English
In this study we try to find out if it is possible to differentiate type of focal cortical dysplasia by features obtained from intracranial EEG. We compare occurrence and rates of three biomarkers present in epilepsy in patients with focal cortical dysplasia type I and II. Case study is made on long term night records of 6 pediatric patients. Detection of interictal epileptiform discharges and high-frequency oscillations is made by automated algorithms, delta brush are marked visually. Position of lesion and electrodes inside were obtained from MRI. In individual rates were not found difference on significant level. No major significance were found, but as promising seem to be ratio inside to outside rates of high-frequency oscillations and presence of delta brush, which were found only in patients with focal cortical dysplasia type II. Keywords: intracranial EEG; focal cortical dysplasia; IED; high frequency oscillations; delta brush Available at various institutes of the ASCR
Case study of intracranial EEG records of patients with focal cortical dysplasia type I and II

In this study we try to find out if it is possible to differentiate type of focal cortical dysplasia by features obtained from intracranial EEG. We compare occurrence and rates of three biomarkers ...

Balach, J.; Ježdík, P.; Čmejla, R.; Kršek, P.; Jiruška, Přemysl
Fyziologický ústav, 2015

Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications
Kročilová, Nikola; Bačáková, Lucie; Pařízek, Martin; Havlíková, Jana; Motarjemi, H.; Molitor, M.; Gabor, R.; Marvan, J.
2015 - English
Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter R-a = 280 nm) or modified by shot blasting and tarnishing A, R-a = 200 nm), vibratory finishing (B, R-a = 100 nm) and vibratory finishing, shot blasting and polishing (C, R-a = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells. Keywords: bone implants; bone tissue engineering; surface roughness; stem cells; liposuction; cell proliferation Available at various institutes of the ASCR
Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications

Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and ...

Kročilová, Nikola; Bačáková, Lucie; Pařízek, Martin; Havlíková, Jana; Motarjemi, H.; Molitor, M.; Gabor, R.; Marvan, J.
Fyziologický ústav, 2015

Analysis of high-frequency activity in electroencephalogram of animal epilepsy model
Havel, T.; Kudláček, J.; Jiruška, Přemysl; Ježdík, P.; Čmejla, R.
2015 - English
High frequency oscillations (HFO) are believed to be a new specific biomarker of epileptogenic tissue. According to the reported findings, we hypothesized that HFOs occur more specific to the epileptogenic tissue. To test this hypothesis, we analyzed intracranial electroencephalograms (iEEG) of three subjects of animal epilepsy model. Overall 180 minutes of iEEG records were processed by the automatic high frequency activity detector and further analyzed. The dominant frequency of each segment was determined and categorized as ripples (80-200 Hz) or fast ripples (200-1000 Hz). Even though overall number of HFO detections predominate in the hemisphere where epileptic focus is located, in comparison of each brain structure separately our hypothesis cannot be fully confirmed. Keywords: epilepsy; animal model; electroencephalogram (EEG); high-frequency oscillations (HFO) Available at various institutes of the ASCR
Analysis of high-frequency activity in electroencephalogram of animal epilepsy model

High frequency oscillations (HFO) are believed to be a new specific biomarker of epileptogenic tissue. According to the reported findings, we hypothesized that HFOs occur more specific to the ...

Havel, T.; Kudláček, J.; Jiruška, Přemysl; Ježdík, P.; Čmejla, R.
Fyziologický ústav, 2015

MORPHOLOGICAL CLUSTERING OF INTERICTAL EPILEPTIFORM DISCHARGES IN INTRACRANIAL ELECTROENCEPHALOGRAPHY
Vlk, Pavel; Janča, R.; Čmejla, R.; Kršek, P.; Marusič, P.; Jiruška, Přemysl
2013 - English
To improve the localizing yield of interictal discharges, identification of specific features of the discharges generated only within epileptogenic region is required. We have developed self-clustering algorithm which discriminates distinct populations of interictal epileptiform discharges according to the morphology of their waveforms. Results demonstrate the ability of the algorithm to separate interictal epileptiform discharges according to their morphological features Keywords: epilepsy; interictal epiletiform discharges; morphological clustering Available at various institutes of the ASCR
MORPHOLOGICAL CLUSTERING OF INTERICTAL EPILEPTIFORM DISCHARGES IN INTRACRANIAL ELECTROENCEPHALOGRAPHY

To improve the localizing yield of interictal discharges, identification of specific features of the discharges generated only within epileptogenic region is required. We have developed ...

Vlk, Pavel; Janča, R.; Čmejla, R.; Kršek, P.; Marusič, P.; Jiruška, Přemysl
Fyziologický ústav, 2013

ANALYSIS OF NON-ENZYMATIC POSTTRANSLATIONAL MODIFICATED (GLYCATED) ALBUMIN BY NANO-LC/MS/MS
Šťastná, Zdeňka; Pataridis, Statis; Sedláková, Pavla; Mikšík, Ivan
2012 - English
Posttranslational modifications of proteins are important reactions, which significantly affect the function of proteins in the organism. In principle, they can be divided into enzymatic and non-enzymatic modifications. Non-enzymatic reactions include glycation (earlier called nonezymatic glycosylation), which plays an important role in the development of chronic complications of diabetes mellitus, uremia, in the process of aging and degeneration of the brain.This work deals with the study of glycated albumins (human serum albumin and bovine serum albumin). Methodologically we used nano-liquid chromatography coupled to Q-TOF mass spectrometer. In vitro modified proteins were cleavaged by trypsin and arising peptides were separated on C18 nano column with trap-column. Peptides and their modifications were analysed by high-resolution Q-TOF mass spectrometer MaXis with precision determination of mass below 2 ppm. We found some modifications of proteins. Besides well known carboxymethyllysine new ones were determined - create mass shift 78, 132 and 218. Origin of these modifications is discussed and possible structure is presented. All found modifications were allocated to the structure of proteins and reactivity to various oxo-compounds was also examined Keywords: non-enzymatic glycation; serum albumin Available at various institutes of the ASCR
ANALYSIS OF NON-ENZYMATIC POSTTRANSLATIONAL MODIFICATED (GLYCATED) ALBUMIN BY NANO-LC/MS/MS

Posttranslational modifications of proteins are important reactions, which significantly affect the function of proteins in the organism. In principle, they can be divided into enzymatic and ...

Šťastná, Zdeňka; Pataridis, Statis; Sedláková, Pavla; Mikšík, Ivan
Fyziologický ústav, 2012

Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images
Michálek, Jan; Čapek, Martin; Mao, X. W.; Kubínová, Lucie
2010 - English
Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction method based on estimating a spatially variable illumination gain, and multiplying acquired CLSM images by the inverse of the estimated gain, is presented. The method does not require any special calibration (reference) images since the gain estimate is extracted from the CLSM image being corrected itself. The proposed approach exploits two types of morphological filters: the median filter and a morphological operator called the upper Lipschitz cover Keywords: CLSM; illumination inhomogeneities Available at various institutes of the ASCR
Application of morphology filters to compensation of lateral illumination inhomogeneities in confocal microscopy images

Due to multiple distortion effects, confocal laser scanning microscopy (CLSM) images of even homogeneous specimen regions suffer from irregular brightness variations. In this paper, a fast correction ...

Michálek, Jan; Čapek, Martin; Mao, X. W.; Kubínová, Lucie
Fyziologický ústav, 2010

Fitting geometrical models to 3D confocal microscopy images using graph cuts iteratively
Janáček, Jiří
2010 - English
A new iterative method for fitting triangulated surfaces and geometric graphs to surfaces and fibre-like structures is based on the calculation of the minimal graph cut in every iteration. The method was used for improvement of the geometric model of muscle fibres surfaces and capillaries in skeletal muscle from 3D confocal image Keywords: graph cut; skeletal muscle; confocal microscopy Available at various institutes of the ASCR
Fitting geometrical models to 3D confocal microscopy images using graph cuts iteratively

A new iterative method for fitting triangulated surfaces and geometric graphs to surfaces and fibre-like structures is based on the calculation of the minimal graph cut in every iteration. The method ...

Janáček, Jiří
Fyziologický ústav, 2010

Elastic registration of biomedical images on CUDA-Supported graphics procesor units
Michálek, Jan; Čapek, Martin; Janáček, Jiří; Kubínová, Lucie
2010 - English
Elastic registration is a task of finding the matching of two images, using geometric and elastic transformations, so that objects in images have the same size, position and orientation. We apply elastic registration in the framework of volume reconstruction, where an object acquired from parallel physical sections is composed and mutual positions of the sections including deformations caused by their cutting have to be found. The method lies in optimizing a functional consisting of two parts: first, discrete total variation as a measure of roughness and, second, L1 norm as a measure of dissimilarity of images. As a parallelizable optimization strategy we apply a potential-based equivalent transformation of a (max,+)-labelling problem. CUDA-based implementation of the described elastic registration algorithm is reasonably fast, requires seconds to minutes of calculations, provides good results and, thus, can be used for practical tasks dealing with alignment of biomedical images Keywords: volume reconstruction; elastic alignment; CUDA Available at various institutes of the ASCR
Elastic registration of biomedical images on CUDA-Supported graphics procesor units

Elastic registration is a task of finding the matching of two images, using geometric and elastic transformations, so that objects in images have the same size, position and orientation. We apply ...

Michálek, Jan; Čapek, Martin; Janáček, Jiří; Kubínová, Lucie
Fyziologický ústav, 2010

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