Yes (164)
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Chemical vapor generation of cadmium: detector comparison
Sagapova, Linda; Štádlerová, Barbora; Svoboda, Milan; Kratzer, Jan; Musil, Stanislav
2021 - English
A chemical vapor generation system was connected to an in-house assembled atomic fluorescence spectrometer (AFS) equipped with a miniature diffusion flame atomizer in order to assess its potential to Cd determination. Subsequently, analytical performance of three spectrometric detectors was compared (AAS, AFS, ICP-MS) with respect to Cd determination after volatile species generation.
Keywords:
chemical vapor generation; cadmium; atomic fluorescence spectrometry
Available in digital repository of the ASCR
Chemical vapor generation of cadmium: detector comparison
A chemical vapor generation system was connected to an in-house assembled atomic fluorescence spectrometer (AFS) equipped with a miniature diffusion flame atomizer in order to assess its potential to ...
Bioluminescence determination of caspase-3/7 in single osteosarcoma cells
Killinger, Michael; Matalová, Eva; Klepárník, Karel
2021 - English
The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate quantity of relevant proteins at cellular level is essential for appropriate interpretation of diagnostic and therapeutic results. Here, we determined the level of active caspase-3/7 in physiological and pathological (cancer) conditions by our miniaturized instrumentation.
Keywords:
single-cell analysis; osteoblast; osteosarcoma
Available in digital repository of the ASCR
Bioluminescence determination of caspase-3/7 in single osteosarcoma cells
The protein heterogeneity at the single-cell level has been recognized to be vital for an understanding of various life processes during animal development. In addition, the knowledge of accurate ...
Separace VRA/RA ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF) pro potřeby kontroly zákazu biologických zbraní
Kubíček, O.; Duša, Filip; Šalplachta, Jiří; Lunerová, K.; Rosenbergová, K.
2021 - English
Metodika popisuje způsob separace bakteriálních vysoce rizikových a rizikových agens (VRA/RA) ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF). Metoda chip-IEF využívá nově vyvinutý unikátní prototyp zařízení pro vertikální isoelektrickou fokusaci v rozbíhavém toku v čipovém provedení, která na rozdíl od dosavadních postupů umožňuje i separaci bakterií. Separace pomocí chip-IEF funguje i pro šetrně inaktivované bakterie, což významně snižuje bezpečnostní rizika při manipulaci se vzorkem potenciálně obsahujícím VRA/RA.
Keywords:
isoelectric focusing; B-agens; chip
Fulltext is available at external website.
Separace VRA/RA ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF) pro potřeby kontroly zákazu biologických zbraní
Metodika popisuje způsob separace bakteriálních vysoce rizikových a rizikových agens (VRA/RA) ze vzorků pomocí isoelektrické fokusace v čipovém formátu (chip-IEF). Metoda chip-IEF využívá nově ...
Photochemical vapor generation of cobalt for detection by inductively coupled plasma mass spectrometry
Vyhnanovský, Jaromír; Musil, Stanislav
2020 - English
Volatile species of cobalt were generated in a flow-injection system, employing a high-efficiency flow-through UV photoreactor and a formic acid based medium. Optimal generation conditions were found as 10% (v/v) formic acid and 4 mol/L ammonium formate at a flow rate of 4 ml/min.
Keywords:
photochemical vapor generation; cobalt; inductively coupled plasma mass spectrometry
Available in digital repository of the ASCR
Photochemical vapor generation of cobalt for detection by inductively coupled plasma mass spectrometry
Volatile species of cobalt were generated in a flow-injection system, employing a high-efficiency flow-through UV photoreactor and a formic acid based medium. Optimal generation conditions were found ...
Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Hlaváček, Antonín; Křivánková, Jana; Foret, František
2020 - English
Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of UCNPs include near-infrared excitation, multiple and narrow emission bands, negligible autofluorescence and high stability, which make UCNPs ideal luminescence label for use in biological and chemical assays. These assays - e.g. upconversion-linked immunosorbent assay, western blot, lateral flow assay, gel electrophoresis, thin layer chromatography - commonly require the scanning of a planar surface with a high spatial resolution and an excellent sensitivity. The availability of commercial equipment is recently limited because of the novelty of the photon-upconversion phenomenon. Therefore, we report on the construction of photon-upconversion laser scanner. The scanner consists of a laser scanning head, which is attached to a xy-moving stage. The scanning head itself is constructed as an epiluminescence detector with excitation wavelength of 976 nm. A CCD array spectroscope is connected to the laser head and serves as a sensitive detector of photon-upconversion luminescence. The scanner possesses a spatial resolution of 200 μm, the scanning rate is up to 57 points per second and the sensitivity reaches down to single photon-upconversion nanoparticle.
Keywords:
photon-upconversion; nanoparticle; laser scanner; hyperspectral imaging
Available in digital repository of the ASCR
Fast and highly sensitive laser scanner for recording photon-upconversion luminiscence from planar surfaces
Photon-upconversion nanoparticles (UCNPs) are lanthanide-doped nanocrystals that can be excited by nearinfrared light and emit photon-upconversion luminescence of shorter wavelengths. Advantages of ...
Photon-upconversion scanner for multiplexed imaging
Hlaváček, Antonín; Křivánková, Jana; Foret, František
2020 - English
In analytical chemistry, multiplexed assays facilitate parallel detection of analytes. The multiplexing reduces the consumption of sample, reagents, and other resources and can be applied for medical diagnostics, the studies of biomolecule interactions, single-cell and singlemolecule assays, environmental monitoring, pathogen detection, multiparameter chemical and biological assays and screening of chemical libraries.
Keywords:
photon-upconversion scanner; photon-upconversion nanoparticles; droplet microfluidics
Fulltext is available at external website.
Photon-upconversion scanner for multiplexed imaging
In analytical chemistry, multiplexed assays facilitate parallel detection of analytes. The multiplexing reduces the consumption of sample, reagents, and other resources and can be applied for medical ...
Photochemical vapour generation of bismuth coupled with atomic fluorescence spectrometry
Štádlerová, Barbora; Vyhnanovský, Jaromír; Dědina, Jiří; Musil, Stanislav
2020 - English
Atomization of bismuthane in the flame-in-gas-shield atomizer by photochemical vapour generation coupled with atomic fluorescence spectrometry.
Keywords:
atomic fluorescence spectrometry; bismuth; photochemical vapour generation
Available in digital repository of the ASCR
Photochemical vapour generation of bismuth coupled with atomic fluorescence spectrometry
Atomization of bismuthane in the flame-in-gas-shield atomizer by photochemical vapour generation coupled with atomic fluorescence spectrometry.
DNA damage induced by occupational exposure to copper oxide nanoparticles
Rössner st., Pavel; Pelcová, D.; Elzeinová, Fatima; Mikuška, Pavel; Večeřa, Zbyněk; Coufalík, Pavel; Vlčková, Š.; Fenclová, Z.; Rössnerová, Andrea
2020 - English
Copper oxide nanoparticles (CuO NPs) have a widespread use in industry, chemistry, in production of electronic devices and as an antimicrobial agent. Although copper is an important biogenic element, CuO NPs are toxic with the ability to induce oxidative stress, apoptosis, cell cycle arrest or DNA damage. For humans, the inhalation route is the most common way of exposure to CuO NPs. In the body, CuO NPs may be either deposited in the lungs, or transported to other organs. Their presence usually causes oxidative stress or inflammatory responses, consequently leading to DNA damage. In this study, we investigated the effect of CuO NPs inhalation on DNA damage in a group of researches conducting animal exposure experiments. The subjects were exposed to various metal oxide nanoparticles, including CuO NPs, by inhalation for an average of 4.9 ± 0.4 years. The average mass concentration of Cu in the air during the experiment was 7.3 ± 3.2 ng/m3. Subjects not exposed to nanoparticles served as a control group. We applied micronucleus assay using Human Pan Centromeric probes to detect DNA damage and to distinguish between the frequency of centromere positive (CEN+) and centromere negative (CEN−) micronuclei (MN) in the binucleated cells. We\ndid not find differences between both groups for either mean MN frequency (10.38 ± 2.50 vs. 11.88 ± 3.01 MN/1000 binucleated cells), or CEN+/CEN- ratio (58%/42% vs. 55%/45%), for the exposed and controls, respectively. In conclusion, inhalation of CuO NPs at this low-level exposure had no effect on chromosomal losses and/or breaks.
Keywords:
copper oxide nanoparticles; inhalation; DNA damage
Available in digital repository of the ASCR
DNA damage induced by occupational exposure to copper oxide nanoparticles
Copper oxide nanoparticles (CuO NPs) have a widespread use in industry, chemistry, in production of electronic devices and as an antimicrobial agent. Although copper is an important biogenic element, ...
Deposition of dried colloidal suspension via nanoelectrospray
Jonas, Vladimír; Týčová, Anna; Přikryl, Jan; Kotzianová, A.; Velebný, V.; Foret, František
2020 - English
Electrospray is a relatively well-established tool for the deposition of nanoparticles. In this work, we use nanoelectrospray (nES) operating at flow rates down to tens of nanoliters per minute. As a result, the formed aerosol consists of very small droplets quickly drying during the flight towards a counter-electrode. Therefore, we obtained deposits of increased homogeneous distribution without an undesirable coffee-ring effect. Moreover, the character of the aerosol plume is only a few millimeters wide, so it allows good control over the deposit localization.
Keywords:
nanoparticles; nanoelectrospray; silver
Fulltext is available at external website.
Deposition of dried colloidal suspension via nanoelectrospray
Electrospray is a relatively well-established tool for the deposition of nanoparticles. In this work, we use nanoelectrospray (nES) operating at flow rates down to tens of nanoliters per minute. As a ...
Chemical vapor generation of cadmium for analytical atomic spectrometry
Sagapova, Linda; Kodríková, Barbora; Svoboda, Milan; Musil, Stanislav; Kratzer, Jan
2020 - English
Chemical vapor generation of cadmium volatile compounds was optimized, several reaction modifiers based on inorganic salts and complexes of CrIII+, CoII+, TiIII+, TiIV+ were tested. Their use resulted in 4-5 times enhancement in sensitivity, reflected also in corresponding increase of generation efficiency and better repeatability. Generation efficiency was determined from a comparison between sensitivities obtained with chemical vapor generation and conventional solution nebulization, both simultaneously coupled with inductively coupled plasma mass spectrometry. The identity of the generated cadmium compounds was identified.
Keywords:
cadmium; chemical vapor generation; atomic absorption spectrometry
Available in digital repository of the ASCR
Chemical vapor generation of cadmium for analytical atomic spectrometry
Chemical vapor generation of cadmium volatile compounds was optimized, several reaction modifiers based on inorganic salts and complexes of CrIII+, CoII+, TiIII+, TiIV+ were tested. Their use resulted ...
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