Number of found documents: 483
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Submerzní kultivace hlenky Schizochytrium spp.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
2016 - Czech
Vývoj kultivace hlenky pro produkci DHA. Definice media, aeračního a míchacího systému, optimální délky kultivace. Testování metod DSP pro maximální výtěžnost produktu. Development of slime mold cultivation for the DHA production. The definition of the medium, the aeration and mixing system, the optimal length of the cultivation. Testing of DSP methods for the maximum product yield. Keywords: mold cultivation; DHA Available at various institutes of the ASCR
Submerzní kultivace hlenky Schizochytrium spp.

Vývoj kultivace hlenky pro produkci DHA. Definice media, aeračního a míchacího systému, optimální délky kultivace. Testování metod DSP pro maximální výtěžnost produktu....

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Vavruška, Tomáš
Mikrobiologický ústav, 2016

Pilotní scale-up of přípravy podpůrných přípravků pro ošetření řepky a kukuřice.
Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Prell, Aleš; Vavruška, Tomáš
2016 - Czech
Pilotní scale-up technologie výroby mikrobiálních pomocných přípravků pro pěstování hospodářských rostlin řepky olejky a kukuřice. Pilot scale-up of production technology of microbial auxiliary fertilizers for agricultural production of oilseed rape and maize. Keywords: auxiliary fertilizers Available at various institutes of the ASCR
Pilotní scale-up of přípravy podpůrných přípravků pro ošetření řepky a kukuřice.

Pilotní scale-up technologie výroby mikrobiálních pomocných přípravků pro pěstování hospodářských rostlin řepky olejky a kukuřice....

Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Prell, Aleš; Vavruška, Tomáš
Mikrobiologický ústav, 2016

ANTIBACTERIAL EFFECT OF SELECTED NANOPARTICLES AS REVEALED BY DOUBLING TIME OF TREATED ANTHOMONAS CAMESTRIS PV. CAMPESTRIS CULTURES
Pečenka, J.; Svobodová, Kateřina; Eichmeier, A.; Baránek, M.
2016 - English
Besides many possibilities of applications of nanoparticles in the field of medicine, diagnostics, molecular biology, bioorganic chemistry or remediation of environment, there is also a potential of employment of nanoparticles as a tool for elimination and control of bacteria invading plant tissue. In this experiment an antibacterial activity of selected nanoparticles based on silver, gold and bimetallic silver/copper was tested on bacteria Xanthomonas campestris pv. campestris (Xcc) (strain 1279a). The strongest inhibitory effect represented by doubling time of treated cultures was measured in the presence of the smallest silver nanoparticles (9 nm) at the highest concentration (5 ppm). Keywords: nanoparticles; Xanthomonas campestris; doubling time Available at various institutes of the ASCR
ANTIBACTERIAL EFFECT OF SELECTED NANOPARTICLES AS REVEALED BY DOUBLING TIME OF TREATED ANTHOMONAS CAMESTRIS PV. CAMPESTRIS CULTURES

Besides many possibilities of applications of nanoparticles in the field of medicine, diagnostics, molecular biology, bioorganic chemistry or remediation of environment, there is also a potential of ...

Pečenka, J.; Svobodová, Kateřina; Eichmeier, A.; Baránek, M.
Mikrobiologický ústav, 2016

UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES
Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
2016 - English
n experiments with farm animals we studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated heterotrophically grown Chlorella biomass significantly increased content of carotenoids in egg yolks of 8 experimental animals already after the first week of supplementation (P<0,01). Compared to 8 control hens these differences remained significantly higher until the end of the eight-week experiment. The average carotenoid concentration was 17,3 mu g per g of yolk in control group and 25,3 mu g per g of yolk in experimental animals (P<0,01). Autotrophically grown Chlorella was supplemented to the 30 gilts one month prior the expected first heat in a dose of 1,5 g per animal per day. These sows entered heat earlier and the number of pregnancy was higher in comparison to the 30 control animals. Other studies observed digestibility of selenium organically bound in Se-enriched Chlorella. The level of selenium in blood, colostrum and also tissues of new born piglets was determined. Group of 8 sows was supplemented with Se-enriched alga during the pregnancy and 3 days after parturition at a dose of 0,3 mg of selenium per 1 kg of feed. The same amount of selenium in the form of sodium selenite was administrated to the control group of 8 individuals. Organic form of selenium significantly increased concentration of Se in blood of sows and colostrum (P<0,05). Piglets born to supplemented sows had also significantly higher level of Se in heart, muscles and liver (P<0.01). The use of fresh Chlorella biomass produced outdoors on a dairy farm was experimentally tested. Location near biogas station enables utilization of flue gas as a source of carbon for algal photosynthesis. The liquid fresh biomass applied to dairy cows during the pregnancy at a dose of 15 g dry mass per cow increased the dry matter content in colostrum from 17,5 g per 100 ml of colostrum in control group to 20,5 g per 100 ml of colostrum in supplemented cows (P<0,05). This pilot experiment demonstrated possibilities of cheaper Chlorella production and its use in ruminant nutrition. Keywords: Chlorella; feed supplement; flue gas utilization Available at various institutes of the ASCR
UNICELLULAR ALGA CHLORELLA AS A FEED SUPPLEMENT, CURRENT EXPERIENCE AND FUTURE PERSPECTIVES

n experiments with farm animals we studied the effect of Chlorella biomass as a feed supplement. In laying hens we found that diet supplemented with 1 % (i.e. 10 g/kg) of dry disintegrated ...

Kotrbáček, V.; Doucha, Jiří; Kuchařová, V.; Doubek, J.
Mikrobiologický ústav, 2016

Optimization of pilot production technology of lactamases and development of Pichia pastoris technology
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - English
Development and optimization of the production of lactamases for pharmaceutical industry. Cultivation technology and down-stream processing. Development of the cultivation and DSP technology of the yeast Pichia pastoris in the pilot scale. Keywords: Pichia cultivation Available at various institutes of the ASCR
Optimization of pilot production technology of lactamases and development of Pichia pastoris technology

Development and optimization of the production of lactamases for pharmaceutical industry. Cultivation technology and down-stream processing. Development of the cultivation and DSP technology of the ...

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2015

Vývoj a optimalizace přípravy podpůrných přípravků pro ošetření řepky a kukuřice.
Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Prell, Aleš
2015 - Czech
Vývoj a scale up technologie výroby mikrobiálních pomocných přípravků pro pěstování hospodářských rostlin řepky olejky a kukuřice. Development and scale-up of production technology of microbial auxiliary fertilizers for agricultural production of oilseed rape and maize. Keywords: auxiliary fertilizers Available at various institutes of the ASCR
Vývoj a optimalizace přípravy podpůrných přípravků pro ošetření řepky a kukuřice.

Vývoj a scale up technologie výroby mikrobiálních pomocných přípravků pro pěstování hospodářských rostlin řepky olejky a kukuřice....

Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek; Prell, Aleš
Mikrobiologický ústav, 2015

Metodika stanovení volného kyanidu (CN-) v průmyslových odpadních vodách
Kaplan, Ondřej; Chmátal, Martin; Veselá, Alicja Barbara; Rinágelová, Anna; Martínková, Ludmila
2015 - Czech
Práce shrnuje aktuální metodiky stanovení CN- zejména v průmyslových odpadních vodách. Je zde dále podrobně popsán způsob stanovení kyanidových iontů po jejich enzymové konverzi na formamid, který se stanoví pomocí HPLC. This work summarizes the current state in the methodology of CN- determination particularly in industrial wastewaters. It also describes in detail the determination of cyanide ions after their enzymatic conversion into formamide, which is determined by HPLC (including the method of preparation of the enzyme). Keywords: cyanide; HPLC; formamide Available on request at various institutes of the ASCR
Metodika stanovení volného kyanidu (CN-) v průmyslových odpadních vodách

Práce shrnuje aktuální metodiky stanovení CN- zejména v průmyslových odpadních vodách. Je zde dále podrobně popsán způsob stanovení kyanidových iontů po jejich enzymové konverzi na formamid, který se ...

Kaplan, Ondřej; Chmátal, Martin; Veselá, Alicja Barbara; Rinágelová, Anna; Martínková, Ludmila
Mikrobiologický ústav, 2015

Optimalizace propagace vinné kvasničné kultury
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - Czech
Vývoj a optimalizace přípravy různých lokálních kultur vinných kvasinek Development and optimization of the production of various cultures of wine yeasts. Keywords: yeast cultivation Available at various institutes of the ASCR
Optimalizace propagace vinné kvasničné kultury

Vývoj a optimalizace přípravy různých lokálních kultur vinných kvasinek


Development and optimization of the production of various cultures of wine yeasts.

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2015

BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS
Fijalkowski, M.; Adach, K.; Marešová, Helena; Kroisová, D.
2015 - English
The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an evaluation of the two systems from the point of view of chemical composition, particle size and structure, ability to form clusters of particles and interactions with selected bacterial systems. Methods used for comparison were: SEM, EDX, TEM, FTIR, ICPOES and bacteriological tests. Husks are standardly contaminated by accompanying ions that are important for plant growth, but undesirable for obtaining a quality product. Rice husk used for the silica isolation were purified by boiling in 10% HCl for 2 hours. Rice husks were then washed with distilled water until pH 7. The dried and purified husks were burned in an oven at 650 degrees C of ramping temperature 10 degrees C/min during 2 hours. The obtained product has a chemical composition analogous to the synthetic product. Biogenic particles reach a size of about 20 nm, about 10 nm synthetic. Both materials have an amorphous structure. Interaction with bacterial systems was performed with Gram-negative bacteria Escherichia coli W3110 strain and Gram-positive bacteria Bacillus cereus. In both cases analogous behaviour was observed depending on the concentration of nanoparticles and reducing the growth rate of cultures of 8% for E. coli and 5% for B. cereus resulted nanoparticles at the concentration of 150 mg/litter of medium. Keywords: Biogenic silica; synthetic silica; chemical composition Available at various institutes of the ASCR
BIOGENIC AND SYNTHETIC AMORPHOUS SILICA - COMPARE AND INTERACTION WITH BACTERIAL SYSTEMS

The aim of the work is to compare the biogenic silica nanoparticles, which have been isolated from rice husks with synthetically produced silica brand Cab-O-Sil LM-150. The comparison is based on an ...

Fijalkowski, M.; Adach, K.; Marešová, Helena; Kroisová, D.
Mikrobiologický ústav, 2015

Příprava biodegradační biomasy s vysokou enzymatickou aktivitou.
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2015 - Czech
Příprava mikrobiální biomasy pro biodegradace znečištěných vod a půd s vysokou enzymatickou aktivitou pro štěpení typických substrátů. Preparation of microbial biomass for biodegradations od polluted waters and soils endowed with a high enzymatic activity for specific substrates. Keywords: microbial production Available at various institutes of the ASCR
Příprava biodegradační biomasy s vysokou enzymatickou aktivitou.

Příprava mikrobiální biomasy pro biodegradace znečištěných vod a půd s vysokou enzymatickou aktivitou pro štěpení typických substrátů....

Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
Mikrobiologický ústav, 2015

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