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Subsidiary reagens for improvement of enzymatic digestion protocol
Laštovičková, Markéta; Bobáľ, P.; Flodrová, Dana; Bobálová, Janette
2016 - anglický
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.
Klíčová slova:
proteomic identifications; MALDI-TOF MS; enzymatic digestion; peptides
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Subsidiary reagens for improvement of enzymatic digestion protocol
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
Čapka, Lukáš; Mikuška, Pavel; Večeřa, Zbyněk
2016 - anglický
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. The system was applied to the determination of\nDCAs in urban aerosols in Brno. The method was verified using a laboratory generated\nstandard aerosol of DCAs. A sampling of aerosol particles on parallel filter was used\nas a reference method.
Klíčová slova:
atmospheric aerosols; dicarboxylic acids; aerosol collector; ion chromatography
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. ...
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří; Roth, Michal
2016 - anglický
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches.
Klíčová slova:
extraction of bioactive proteins; MALDI-TOF/TOF mass spectrometry; Sambucus nigra L. branches; SDS-PAGE; PHWE
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of ...
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Večeřová, Kristýna; Mikuška, Pavel; Oravec, Michal; Kozáčiková, Michaela; Pompeiano, Antonio; Coufalík, Pavel; Urban, Otmar
2016 - anglický
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week exposure to airborne CdONPs of ecologically\nrelevant size and concentration significantly reduced photosynthetic light use efficiency\nin plants as well as contents of photosynthetic pigments and saccharides. In contrary,\namino acid content was increased under CdONPs. These changes were further\nmodulated by previous growth temperature.
Klíčová slova:
Picea abies; terrestrial ecosystems; CdO nanoparticles
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week ...
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2016 - anglický
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment.
Klíčová slova:
capillary electrophoresis; electrospray-ionization mass spectrometric detection; chlorophenols; sulfonamides
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic ...
A novel nanospray liquid junction interface for versatile CE-MS
Křenková, Jana; Haselberg, R.; Somsen, G. W.; Foret, František
2016 - anglický
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a hybrid microfabricated plastic liquid junction sprayer allowing to perform analysis in standard (30-100 micro m ID) separation capillaries. The interface incorporates a self-aligning port for coupling of the separation capillary as well as ports for automated flushing of both the liquid junction and the separation capillary. The interface permits performing CE both with and without electroosmotic flow. The use of large bore capillaries allows injection and preconcentration of larger sample volumes, favoring detection sensitivity. Different compositions of the spray liquid and BGE can be used when needed.
Klíčová slova:
capillary electrophoresis; mass spectrometry; liquid junction interface
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
A novel nanospray liquid junction interface for versatile CE-MS
In this work we present a new polyimide-based liquid junction nanospray interface, combining the advantages of sheath flow and sheathless designs. The liquid junction interface was prepared as a ...
Determination of levoglucosan in urban aerosol
Coufalík, Pavel; Mikuška, Pavel; Čmelík, Richard
2016 - anglický
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by LC-MS.
Klíčová slova:
monosaccharide anhydrides; urban aerosol; levoglucosan
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Determination of levoglucosan in urban aerosol
The main aim of this work was a development of the analytical method for levoglucosan\ndetermination including direct collection of aerosol into water phase, preconcentration,\nand determination by ...
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Brtko, J.; Toporová, L.; Macejová, D.; Bialešová, L.; Flodrová, Dana; Bobálová, Janette
2016 - anglický
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Klíčová slova:
retinoic acid; retinoid receptors; protein pattern
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Effect of two retinoic acid isomers on the expression of selected nuclear retinoid receptors and protein pattern in human MCF-7 breast cancer cell line
Effect of two retinoic acid isomers on RARalpha, RARgamma, RXRalpha, RXRbeta expression and protein profile in human MCF-7 breast cancer line were investigated.
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Ledvina, Vojtěch; Klepárník, Karel
2016 - anglický
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during apoptosis and after their widespread activation, the process\ncannot be reversed. To analyze caspase-3/7 activation within single cells, a miniaturized\ndevice for parallel analysis of eight samples was developed. The assay is based on the\nmodified luciferin-firefly luciferase bioluminescence (BL) system. Individual\nsuspended cells were collected and transferred into detection microvials using a\nmicromanipulator. The bioluminescence was detected using a photon counting head\nwith cooled photcathode. The LOD suitable for detection of active caspase-3/7 in both\napoptotic and non-apoptotic cells was reached.
Klíčová slova:
single-cell analysis; apoptotic cells; non-apoptotic cells
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Parallel single-cell analysis of active caspase-3/7 in apoptotic and non-apoptotic cells
Caspases are proteases that play key role in the process of apoptosis, the programmed\ncell death. Among them, caspase-3 and -7 are main executioner caspases that cleave\nmany vital proteins during ...
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
Lačná, J.; Foret, František; Kubáň, Petr
2016 - anglický
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new CE-LIF system. Then the derivatization process\nof MDA by TBA was optimized. The ability to use this method for the detection of\nMDA in a biological samples, such as EBC and plasma, was also demonstrated. For the\nfirst time it was possible to detect MDA in EBC samples by CE. Other biomarkers are\nnow being studied.
Klíčová slova:
CE-LIF; malondialdehyde; biomarkers; lipids
Plné texty jsou dostupné v digitálním repozitáři Akademie Věd.
Novel, versatile capillary electrophoresis instrument with laser induced fluorescence for analysis of various lipid peroxidation biomarkers
In this work we have developed a new sensitive method for quantitation of MDA in\nbiological samples by CE-LIF with LOD about 1.7 nM. First part of the work was\ndevoted to the construction of a new ...
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