Genetic and epigenetic acpects of sex determination
Janoušek, Bohuslav; Hodurková, Jaromíra; Žlůvová, Jitka; Lengerová, Martina; Hobza, Roman; Nix, Tomáš; Vyskot, Boris
2002 - English The article concentrates on the latest knowledge concerning the role of epigenetic mechanisms in sex determination in model dioecious species Silene latifolia. Results suggest that the locus suppressing development of female organs in male plants is hypersensitive to the action of agents influencing level of DNA methylation (5-azacytidine) and histone acetylation (trichostatin A). According to the results obtained via inter-specific hybridisation and the ability of the smut Ustilago violacea to activate development of male organs in female plants, it can be deduced that there is a locus suppressing male organ development in standard female plants. Function of this locus is not influenced by the drugs mentioned above. Keywords: Silene latifolia; Ustilago violacea; sex determination Available at various institutes of the ASCR Genetic and epigenetic acpects of sex determination

The article concentrates on the latest knowledge concerning the role of epigenetic mechanisms in sex determination in model dioecious species Silene latifolia. Results suggest that the locus ...

Janoušek, Bohuslav; Hodurková, Jaromíra; Žlůvová, Jitka; Lengerová, Martina; Hobza, Roman; Nix, Tomáš; Vyskot, Boris
Biofyzikální ústav, 2002

Impedance measurements of nucleic acids and self-assembled layers of their components
Vetterl, Vladimír; Jelen, František; Dražan, Viktor; Strašák, Luděk; Hasoň, Stanislav; Dvořák, Jakub
2002 - English The kinetics of structural changes of cytidine adlayers on the mercury electrodes were investigated by means of current and capacitance transients using the potential step technique. Keywords: nucleic acids; electochemical impedance; mercury electrodes Available at various institutes of the ASCR Impedance measurements of nucleic acids and self-assembled layers of their components

The kinetics of structural changes of cytidine adlayers on the mercury electrodes were investigated by means of current and capacitance transients using the potential step technique.

Vetterl, Vladimír; Jelen, František; Dražan, Viktor; Strašák, Luděk; Hasoň, Stanislav; Dvořák, Jakub
Biofyzikální ústav, 2002

Structure and evolution of plant sex chromosomes: The use of BAC library
Kejnovský, Eduard; Lengerová, Martina; Hobza, Roman; Široký, Jiří; Vyskot, Boris
2002 - English Sex chromosomes of plant species Silene latifolia are the ideal model for evolutionary biology studies because they represent early stages of X and Y chromosomes history. We used fluorescence in situ hybridization (FISH) with bacterial artificial chromosome (BAC) clones in studies of chromosome structure to demonstrate that a majority of genes are located in subtelomeric regions of all chromosomes. Our results also indicate the absence of accumulation of main classes of repetitive sequences - retroelements and centromeric tandem repeats - in non-recombining parts of the sex chromosomes. Keywords: sex chromosomes; BAC-FISH; Silene latifolia Available at various institutes of the ASCR Structure and evolution of plant sex chromosomes: The use of BAC library

Sex chromosomes of plant species Silene latifolia are the ideal model for evolutionary biology studies because they represent early stages of X and Y chromosomes history. We used fluorescence in situ ...

Kejnovský, Eduard; Lengerová, Martina; Hobza, Roman; Široký, Jiří; Vyskot, Boris
Biofyzikální ústav, 2002

Defects of pollen development in transgenic tobacco
Dubová, J.; Hájková, M.; Brzobohatý, Břetislav
2001 - English Keywords: pollen development; beta-glucosidase; transgenic plants Available at various institutes of the ASCR Defects of pollen development in transgenic tobacco

Dubová, J.; Hájková, M.; Brzobohatý, Břetislav
Biofyzikální ústav, 2001

Identification of a cysteine residue conferring sensitivity of a maize beta-glucosidase to silver ion and alkylation treatment
Slaný, M.; Zouhar, J.; Brzobohatý, Břetislav
2001 - English Keywords: Cysteine residues; enzyme activity; beta-glucosidase Available at various institutes of the ASCR Identification of a cysteine residue conferring sensitivity of a maize beta-glucosidase to silver ion and alkylation treatment

Slaný, M.; Zouhar, J.; Brzobohatý, Břetislav
Biofyzikální ústav, 2001

Expression and single-step purification of the signal receiver domain of CKI1, a putative cytokinin receptor from Arabidopsis thaliana
Borkovcová, Petra; Zouhar, J.; Hejátko, Jan; Brzobohatý, Břetislav
2001 - English Keywords: CKI1 receiver domain; affinity chromatography; purification Available at various institutes of the ASCR Expression and single-step purification of the signal receiver domain of CKI1, a putative cytokinin receptor from Arabidopsis thaliana

Borkovcová, Petra; Zouhar, J.; Hejátko, Jan; Brzobohatý, Břetislav
Biofyzikální ústav, 2001

Cytogenetics of Retinoblastoma
Bártová, Eva; Gajová, H.; Kozubek, Stanislav; Jirsová, Pavla; Kozubek, Michal
2001 - English The study on the molecular cytogenetics of human retinoblastoma Keywords: retinoblastoma; cytogenetics Available at various institutes of the ASCR Cytogenetics of Retinoblastoma

The study on the molecular cytogenetics of human retinoblastoma

Bártová, Eva; Gajová, H.; Kozubek, Stanislav; Jirsová, Pavla; Kozubek, Michal
Biofyzikální ústav, 2001

Topology and Structure of Dystrophin Gene on Active and Inactive Chromosome X in Human lymphocytes
Falk, Martin; Lukášová, Emilie; Kozubek, Stanislav; Kozubek, Michal
2001 - English Study on the topology and structure of chromosome X and the dystrophin gene in human lymphocytes Keywords: chromosome X; human lymphocytes Available at various institutes of the ASCR Topology and Structure of Dystrophin Gene on Active and Inactive Chromosome X in Human lymphocytes

Study on the topology and structure of chromosome X and the dystrophin gene in human lymphocytes

Falk, Martin; Lukášová, Emilie; Kozubek, Stanislav; Kozubek, Michal
Biofyzikální ústav, 2001

Relationship between Chromosome Structure and Arrangement in the Interphase Nuclei and the Induction of Exchange Aberrations by Ionising Radiation
Cafourková, Alena; Lukášová, Emilie; Kozubek, Stanislav; Kozubek, Michal; Koutná, I.; Jirsová, Pavla; Bártová, Eva
2001 - English Ionising radiation-induced chromosome aberations in relation to the structure of chromosomes and interphase nuclei Keywords: interphase nuclei; chromosome structure; exchange aberrations Available at various institutes of the ASCR Relationship between Chromosome Structure and Arrangement in the Interphase Nuclei and the Induction of Exchange Aberrations by Ionising Radiation

Ionising radiation-induced chromosome aberations in relation to the structure of chromosomes and interphase nuclei

Cafourková, Alena; Lukášová, Emilie; Kozubek, Stanislav; Kozubek, Michal; Koutná, I.; Jirsová, Pavla; Bártová, Eva
Biofyzikální ústav, 2001

Platinum Compounds. From DNA Modifications to Cancer Chemotherapy
Brabec, Viktor
2001 - English The paper deals with DNA modifications and cancer chemotherapy based on platinum compounds Keywords: platinum; DNA; cancer chemotherapy Available at various institutes of the ASCR Platinum Compounds. From DNA Modifications to Cancer Chemotherapy

The paper deals with DNA modifications and cancer chemotherapy based on platinum compounds

Brabec, Viktor
Biofyzikální ústav, 2001