No (505)
Yes (3)
Number of found documents:
508
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to
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Martínková, Ludmila; Kaplan, Ondřej; Veselá, Alicja Barbara
2014 - English
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. The enzymes differ from each other in substrate specificity, temperature optimum, enantioselectivity (arylacetonitrilases) etc. Preferential substrates are phenylacetonitrile, (R,S)-mandelonitrile, benzonitrile or cyanopyridines (depending on the enzyme).
Keywords:
Nitrilase; Expression; Purification
Available at various institutes of the ASCR
Seeking Novel Nitrilase Enzyme (Nitrile Aminohydrolase, EC 3.5.5.1)
Our research group has created a library of novel nitrilases. In general, the enzymes are available from heterologous producers in amounts ranging from 10 to 20 mg of protein exhbiting 25-50 % purity. ...
Gut microbiome and colorectal cancer
Tlaskalová-Hogenová, Helena; Klimešová, Klára; Zákostelská, Zuzana; Kverka, Miloslav; Hornová, Michaela; Vannucci, Luca; Štěpánková, Renata; Hudcovic, Tomáš; Kozáková, Hana; Rossmann, Pavel
2014 - English
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the effects of microbiota on the host.
Keywords:
gut; colorectal; cancer
Available at various institutes of the ASCR
Gut microbiome and colorectal cancer
The work deals with metagenomic approaches that are currently being used to decipher the genome of the microbiota (microbiome), and, in parallel, functional studies are being performed to analyze the ...
DNA sequencing
Felsberg, Jürgen
2014 - Czech
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out. Provedení vědecko-technických prací, spočívajících v sekvenování DNA podle zadání objednatele. Dále jsou prováděny všechny potřebné analýzy nukleových kyselin nutné k sekvenování DNA.
Keywords:
DNA sequencing
Available at various institutes of the ASCR
DNA sequencing
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
Vývoj technologií lyopfilizace buněčných lyzátů
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Keywords:
lyophyllization; cell content; biotechnology
Available at various institutes of the ASCR
Vývoj technologií lyopfilizace buněčných lyzátů
Development and optimization of lyophilization methods of the cell content avoiding loss of functionality.
Verification of the effects of substances ex vivo in a mouse experimental model
Fišerová, Anna
2014 - Czech
The subject of the contract was to test the adjuvant activity of newly synthesized compounds derived from the original preparations of normuramyl glycopeptides. Předmětem smluvního výzkumu bylo vyšetření adjuvantní aktivity nově syntetizovaných látek odvozených od původních preparátů normuramylových glykopeptidů.
Keywords:
flow cytometry; NK activity; proliferation; cytokines
Available at various institutes of the ASCR
Verification of the effects of substances ex vivo in a mouse experimental model
The subject of the contract was to test the adjuvant activity of newly synthesized compounds derived from the original preparations of normuramyl glycopeptides.
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Brennerová, Mária
2014 - English
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria from technological solutions, and taxonomical identification through sequencing of 16S ribosomal RNA genes and searching for affiliations with bacterial relatives in the NCBI website.
Keywords:
viscose; technological solutions; bacterial isolates; identification
Available at various institutes of the ASCR
Microbial and molecular biological analysis of technological solutions from Glanzstoff Bohemia s.r.o
Main goal of the study was identification of the bacterial contaminations during the production of high-tenacity viscose filaments. The work describes microbial cultivations, isolation of bacteria ...
DNA sequencing
Felsberg, Jürgen
2014 - Czech
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out. Provedení vědecko-technických prací, spočívajících v sekvenování DNA podle zadání objednatele. Dále jsou prováděny všechny potřebné analýzy nukleových kyselin nutné k sekvenování DNA.
Keywords:
DNA sequencing
Available at various institutes of the ASCR
DNA sequencing
Performance of scientific and technical work largely consisting of DNA sequencing according to client specification. In adition all DNA analysis necessery for DNA sequencing are carried out.
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - English
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Keywords:
enzyme production; bacterial production; submergeed aerobic cultivation
Available at various institutes of the ASCR
Vývoj kultivačních technologií výroby intracelulárních alkoholdehydrogenáz
Development and optimization of pilot cultivations and DSP of intracellular recombinant enzyme productions.
Development of production technology of bacterial polymers
Prell, Aleš; Kujan, Petr; Sobotka, Miroslav; Šafář, Hynek
2014 - Czech
Development and scale-up of technology of biopolymers' preparation by bacterial cultivation using waste material. Usage in preparation of biodegradable plastics. Vývoj a scale up technologie přípravy biopolymerů bakteriální kultivací na odpadní surovině. Využití pro přípravu biodegradovatelných plastů.
Keywords:
microbial production; downstream processing; scale-up
Available at various institutes of the ASCR
Development of production technology of bacterial polymers
Development and scale-up of technology of biopolymers' preparation by bacterial cultivation using waste material. Usage in preparation of biodegradable plastics.
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
Kozáková, Hana
2014 - English
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete microflora.
Keywords:
flies; lactobacili; mice
Available at various institutes of the ASCR
Host interactions and lactobacilli: from fly model to mouse model/ Role of the intestinal lactobacilli in growth promoting in juvenile development
We confirmed the hypothesis that the intestinal flora supports juvenile growth in mice, and that the bacterial isolate is capable and mice recapitulate promote growth, similarly as complete ...
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