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CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis
Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel
2016 - English
This year the meeting included full texts of 32 oral and 78 poster presentations delivered by participants from 17 countries. The attached conference proceedings, edited by highly cited scientists, documents the quality of the meeting. Similarly to the previous years the lectures were given by outstanding, highly cited, scientists. The list of speakers as well as more detailed information about the conference can be found on its permanent web at www.ce-ce.org. Keywords: bioanalysis; separation; biotechnology Fulltext is available at external website.
CECE 2016. 13th International Interdisciplinary Meeting on Bioanalysis

This year the meeting included full texts of 32 oral and 78 poster presentations delivered by participants from 17 countries. The attached conference proceedings, edited by highly cited scientists, ...

Foret, František; Křenková, Jana; Drobníková, Iveta; Klepárník, Karel
Ústav analytické chemie, 2016

Subsidiary reagens for improvement of enzymatic digestion protocol
Laštovičková, Markéta; Bobáľ, P.; Flodrová, Dana; Bobálová, Janette
2016 - English
In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications. Keywords: proteomic identifications; MALDI-TOF MS; enzymatic digestion; peptides Available in digital repository of the ASCR
Subsidiary reagens for improvement of enzymatic digestion protocol

In this report, we demonstrate the influence of hydrogen peroxide, phenol and\nacetonitrile on enzymatic digestion of proteins accomplished during common bottomup\nproteomic identifications.

Laštovičková, Markéta; Bobáľ, P.; Flodrová, Dana; Bobálová, Janette
Ústav analytické chemie, 2016

Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit
Čapka, Lukáš; Mikuška, Pavel; Večeřa, Zbyněk
2016 - English
A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. The system was applied to the determination of\nDCAs in urban aerosols in Brno. The method was verified using a laboratory generated\nstandard aerosol of DCAs. A sampling of aerosol particles on parallel filter was used\nas a reference method. Keywords: atmospheric aerosols; dicarboxylic acids; aerosol collector; ion chromatography Available in digital repository of the ASCR
Determination of dicarboxylic acids in atmospheric aerosols using ion chromatography with on-line connected preconcentration unit

A method for the fast and sensitive detection of dicarboxylic acids in atmospheric\naerosols was optimized. The method is based on the online sampling and subsequent\nanalysis with ion chromatography. ...

Čapka, Lukáš; Mikuška, Pavel; Večeřa, Zbyněk
Ústav analytické chemie, 2016

Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches
Hohnová, Barbora; Šalplachta, Jiří; Roth, Michal
2016 - English
Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of bioactive proteins in Sambucus nigra L. branches. Keywords: extraction of bioactive proteins; MALDI-TOF/TOF mass spectrometry; Sambucus nigra L. branches; SDS-PAGE; PHWE Available in digital repository of the ASCR
Investigation of bioactive proteins in pressurized water extract of Sambucus nigra L. branches

Investigation of high-value substances in plant materials has been of increasing interest\nin last years. In this study, PHWE, SDS-PAGE and MALDI-TOF techniques were\nemployed for investigation of ...

Hohnová, Barbora; Šalplachta, Jiří; Roth, Michal
Ústav analytické chemie, 2016

Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection
Malá, Zdeňka; Gebauer, Petr; Boček, Petr
2016 - English
This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic focusing on an inverse electromigration dispersion\n(EMD) gradient. The technique is based on a robust electrolyte system providing a\ngradient of required properties, suitable for routine analyses of trace amounts of weak\nacids with pKa values between approx. 6.5 and 9. Examples analyses of several\nchlorophenols and sulfonamides are presented, with LODs around 3x10-9 M for spiked\ndrinking water without any sample pretreatment. Keywords: capillary electrophoresis; electrospray-ionization mass spectrometric detection; chlorophenols; sulfonamides Available in digital repository of the ASCR
Highly sensitive analysis of chlorophenols and sulfonamides in waters by electrophoretic focusion on inverse electromigration dispersion gradient with ESI-MS detection

This work presents a new methodology for high-sensitivity analyses by capillary\nelectrophoresis (CE) with electrospray-ionization mass spectrometric (ESI-MS)\ndetection, based on electrophoretic ...

Malá, Zdeňka; Gebauer, Petr; Boček, Petr
Ústav analytické chemie, 2016

Development of microfluidic device for droplet generation and microparticle encapsulation
Křivánková, Jana; Foret, František
2016 - English
Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is divided into numerous droplets surrounded by a\ncontinuous immiscible fluid phase. Generated droplets serve then as\nvessels/microcapsules for delivering drugs, nutrients as well as for encapsulating of\nbiologically active particles and cells.\nDescribed experimental study deals with the development of a practical dropletbased\nmicrofluidic chip useful for production of monodisperse water/oil emulsion and\nfor encapsulation of thin-metal magnetic microsheets. Keywords: microfluidic device; microparticle encapsulation Available in digital repository of the ASCR
Development of microfluidic device for droplet generation and microparticle encapsulation

Microfluidic devices combined with various bioanalytical and optical sensors proved\ntheir potential in chemical, biological, and biomedicine applications. At a droplet\nmicrofluidic device a fluid is ...

Křivánková, Jana; Foret, František
Ústav analytické chemie, 2016

Combined effect of temperature and CdO nanoparticles treatment on Picea abies
Večeřová, Kristýna; Mikuška, Pavel; Oravec, Michal; Kozáčiková, Michaela; Pompeiano, Antonio; Coufalík, Pavel; Urban, Otmar
2016 - English
Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week exposure to airborne CdONPs of ecologically\nrelevant size and concentration significantly reduced photosynthetic light use efficiency\nin plants as well as contents of photosynthetic pigments and saccharides. In contrary,\namino acid content was increased under CdONPs. These changes were further\nmodulated by previous growth temperature. Keywords: Picea abies; terrestrial ecosystems; CdO nanoparticles Available in digital repository of the ASCR
Combined effect of temperature and CdO nanoparticles treatment on Picea abies

Three-years old seedlings of Picea abies were pre-treated under two different\ntemperatures (20x35 °C) and subsequently exposed to elevated concentration of CdO\nnanoparticles (CdONPs). Two-week ...

Večeřová, Kristýna; Mikuška, Pavel; Oravec, Michal; Kozáčiková, Michaela; Pompeiano, Antonio; Coufalík, Pavel; Urban, Otmar
Ústav analytické chemie, 2016

Charakterizace organických sloučenin v PM1 aerosolu v Kladně v zimním období 2016
Křůmal, Kamil; Mikuška, Pavel; Večeřa, Zbyněk
2016 - Czech
Organické sloučeniny a markery používané pro identifikaci hlavních emisních zdrojů byly měřeny v PM1 aerosolu v malém městě Kladně-Švermově. Nejvyšší koncentrace byly pro anhydridy monosacharidů a pryskyřičné kyseliny (spalování biomasy). Dalšími analyzovanými skupinami organických sloučenin a markerů byly polycyklické aromatické uhlovodíky (emise z dopravy a nedokonalého spalování), hopany (spalování uhlí, doprava), sterany (doprava), sacharidy a alkany. In this work the organic compounds and organic markers used for the identification of main aerosol sources were measured in PM1 aerosol in a small town (Kladno-Švermov) near Prague. Monosaccharide anhydrides and resin acids (emissions from biomass combustion) were found especially. Polycyclic aromatic hydrocarbons (emissions from traffic and incomplete combustion), hopanes (traffic, coal combustion), steranes (traffic), saccharides and alkanes were other groups of analysed organic compounds. Keywords: organic tracer; urban aerosol; PM1 Available in digital repository of the ASCR
Charakterizace organických sloučenin v PM1 aerosolu v Kladně v zimním období 2016

Organické sloučeniny a markery používané pro identifikaci hlavních emisních zdrojů byly měřeny v PM1 aerosolu v malém městě Kladně-Švermově. Nejvyšší koncentrace byly pro anhydridy monosacharidů a ...

Křůmal, Kamil; Mikuška, Pavel; Večeřa, Zbyněk
Ústav analytické chemie, 2016

SMV-2016-719: Detekce a identifikace zdrojů nanočástic produkovaných činností vojenské techniky
Večeřa, Zbyněk
2016 - Czech
V rámci studie, která probíhala ve vojenském výcvikovém prostoru „Vyškov“ a na trenažerech Univerzity obrany v Brně, se sledovaly zdroje nanočástic produkovaných různými druhy vojenské techniky. Předběžné výsledky ukazují, že zdrojem nanočástic jsou zejména výfukové zplodiny motorů pohánějících studovanou vojenskou techniku a povýstřelové zplodiny. Možný vliv nanočástic na obsluhu vojenské techniky se v současnosti vyhodnocuje. In the study, which took place in a military training area "Vyškov" and simulators University of Defence, we monitored and identified the sources nanoparticles produced various kinds of military equipment. Preliminary results indicate that the source of nanoparticles are especially exhaust gases of engines powering military equipment and studied after shoot fumes. Potential impact of nanoparticles on the service staff of military equipment is currently being evaluated. Keywords: nanoparticles Available on request at various institutes of the ASCR
SMV-2016-719: Detekce a identifikace zdrojů nanočástic produkovaných činností vojenské techniky

V rámci studie, která probíhala ve vojenském výcvikovém prostoru „Vyškov“ a na trenažerech Univerzity obrany v Brně, se sledovaly zdroje nanočástic produkovaných různými druhy vojenské techniky. ...

Večeřa, Zbyněk
Ústav analytické chemie, 2016

Metodika aplikace esenciálních olejů a jejich složek k dosažení fungiastického účinku v depozitářích papírových artefaktů ohrožených mikrobiálním napadením vlivem vysoké relativní vlhkosti
Milichovský, M.; Večeřa, Zbyněk; Neuvirt, J.
2016 - Czech
Metodika se týká ochrany artefaktů na papíře s použitím esenciálních olejů. Je souhrnem znalostí, zásad a postupů, jak tyto látky nebo jejich analogy připravené uměle používat a vyvarovat se tak zbytečných chyb až negativních dopadů na ochraňované lignocelulózové materiály. Methodology concerns the protection of artifacts on paper using essential oils. It is the sum of knowledge, policies and procedures, as these substances or their analogs artificially prepared to use so as to avoid unnecessary mistakes to be protected negative impact on lignocellulosic materials. Keywords: essential oils; archived paper materials; relative humidity; microbial attack Available on request at various institutes of the ASCR
Metodika aplikace esenciálních olejů a jejich složek k dosažení fungiastického účinku v depozitářích papírových artefaktů ohrožených mikrobiálním napadením vlivem vysoké relativní vlhkosti

Metodika se týká ochrany artefaktů na papíře s použitím esenciálních olejů. Je souhrnem znalostí, zásad a postupů, jak tyto látky nebo jejich analogy připravené uměle používat a vyvarovat se tak ...

Milichovský, M.; Večeřa, Zbyněk; Neuvirt, J.
Ústav analytické chemie, 2016

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