Number of found documents: 75
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Odborná zpráva o postupu prací a dosažených výsledcích za rok 2016 v rámci projektu TA04011345
Hrůzová, D.; Matějka, R.; Rosina, J.; Zárubová, Jana; Filová, Elena
2016 - Czech
Zpráva shrnuje postupy realizace projektu TA04011345 Cévní protézy o malém průměru osídlované endotelovými a kmenovými buňkami kostní dřeně v bioreaktoru v rámci etapy 2016. Hlavními body jsou: optimalizace decelularizačního postupu pro získání standardizovaného materiálu pro buněčné osídlení, postup trojrozměrného osídlování cévní protézy - endotelizace, vývoj systému bioreaktoru, mechanické zkoušky, chemické potahování protézy, získání buněk od pacientů a jejich charakterizace. Report sums progress of project TA04011345 in 2016 phase. Main topics are: optimization of automated decellularization, bioreactor design for 3D re-endothelisation, mechanical evaluation tests, chemical modifications, cell harvesting from patients and their characterisation. Keywords: annual report; TACR Available at various institutes of the ASCR
Odborná zpráva o postupu prací a dosažených výsledcích za rok 2016 v rámci projektu TA04011345

Zpráva shrnuje postupy realizace projektu TA04011345 Cévní protézy o malém průměru osídlované endotelovými a kmenovými buňkami kostní dřeně v bioreaktoru v rámci etapy 2016. Hlavními body jsou: ...

Hrůzová, D.; Matějka, R.; Rosina, J.; Zárubová, Jana; Filová, Elena
Fyziologický ústav, 2016

Odborná zpráva o postupu prací a dosažených výsledcích za rok 2015 v rámci projektu TA04011345
Hrůzová, D.; Matějka, R.; Filová, Elena
2015 - Czech
Zpráva shrnuje postupy realizace projektu TA04011345 Cévní protézy o malém průměru osídlované endotelovými a kmenovými buňkami kostní dřeně v bioreaktoru v rámci etapy 2014. Hlavními body jsou: optimalizace odběru prasečích cév vhodných pro deceluarizaci; optimalizace postupů deceluarizace; optimalizace podmínek při dynamické kultivaci endotelu, kmenových buněk a jejich kokultur; návrh komory a perfuzního systému pro 3D dynamickou kultivaci v deceluarizovaných nosičích Report sums progress of project TA04011345. Main topics are: optimization of porcine artery harvesting, optimization of decellularization process, optimization of dynamic cultivation of endothelium, stem cells and their co-cultures, design of cultivation chamber and perfusion system used for 3D dynamic cultivation in decellularized tubular scaffolds Keywords: annual report; TACR Available at various institutes of the ASCR
Odborná zpráva o postupu prací a dosažených výsledcích za rok 2015 v rámci projektu TA04011345

Zpráva shrnuje postupy realizace projektu TA04011345 Cévní protézy o malém průměru osídlované endotelovými a kmenovými buňkami kostní dřeně v bioreaktoru v rámci etapy 2014. Hlavními body jsou: ...

Hrůzová, D.; Matějka, R.; Filová, Elena
Fyziologický ústav, 2015

Polymeric nanofibrous scaffolds reinforced with diamond and ceramic nanoparticles for bone tissue engineering
Bačáková, Lucie; Pařízek, Martin; Staňková, Ľubica; Novotná, Katarína; Douglas, T.E.L.; Brady, M. A.; Kromka, Alexander; Potocký, Štěpán; Stránská, D.
2015 - English
Three types of nanofibrous scaffolds were prepared by electrospining: (1) poly(lactide-co-glycoside) (PLGA) scaffolds reinforced with 23 wt.% of diamond nanoparticles (DNPs), (2) poly(L-lactide) (PLLA) scaffolds with DNPs in concentration ranging from from 0.4 wt.% to 12.3 wt.%, and (3) PLLA scaffolds with 5 wt.% or 15 wt.% of hydroxyapatite (HAp) nanoparticles. The diameter of the nanofibers ranged between 160 and 729 nm. The nanofibers with nanoparticles were thicker and the void spaces among them were smaller. Mechanical properties of the nanoparticle-loaded scaffolds were better, as demonstrated by a rupture test in scaffolds with DNPs and by a creep behavior test in scaffolds with HAp. On PLGA scaffolds with DNPs, the human osteoblast-like MG-63 cells adhered in similar numbers and grew with similar kinetics as on pure PLGA scaffolds. Human bone marrow mesenchymal stem cells grew faster and reached higher population densities on PLGA-DNP scaffolds. However, on PLLA-based scaffolds, the activity of mitochondrial enzymes and concentration of osteocalcin in MG-63 cells decreased with increasing DNP concentration. On the other hand, the metabolic activity of MG-63 cells and content of osteocalcin in these cells were positively correlated with the HAp concentration in PLLA scaffolds. Thus, PLGA nanofibers with 23 wt% of DNPs and PLLA nanofibers with 5 and particularly 15 wt.% of HAp seem to be promising for bone tissue engineering. Keywords: material nanostructure; nanofibers; osteogenic cells; bone implants; regenerative medicine Available at various institutes of the ASCR
Polymeric nanofibrous scaffolds reinforced with diamond and ceramic nanoparticles for bone tissue engineering

Three types of nanofibrous scaffolds were prepared by electrospining: (1) poly(lactide-co-glycoside) (PLGA) scaffolds reinforced with 23 wt.% of diamond nanoparticles (DNPs), (2) poly(L-lactide) ...

Bačáková, Lucie; Pařízek, Martin; Staňková, Ľubica; Novotná, Katarína; Douglas, T.E.L.; Brady, M. A.; Kromka, Alexander; Potocký, Štěpán; Stránská, D.
Fyziologický ústav, 2015

Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization
Doubková, Martina; Bačáková, Lucie; Pařízek, Martin; Vandrovcová, Marta; Musílková, Jana; Lisá, Věra; Gabor, R.; Marvan, J.
2015 - English
Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is known to enhance osseointegration. The aim of this study is to evaluate the adhesion, growth and osteogenic differentiation of human osteoblast-like SAOS-2 cells on Ti-6Al-4V samples anodized in electrolytes composed of a stable volume of KOH (336.48 g/l) and variable volumes of liquid glass (124.47 g/l, 84,12 g/l and 37.38 g/l; samples s5, s6 and s7, respectively). Non-anodized Ti-6Al-4V samples, cell culture polystyrene (PS) and microscopic glass coverslips served as control materials. On days 2 and 4 after seeding, the cell number did not differ significantly among the tested samples. However, on day 7, the cell number on s6 samples reached the lowest values, which could be attributed to a non-homogeneous TiO2 film on s6 samples formed during anodization. Nevertheless, the osteogenic differentiation, estimated by the intensity of fluorescence of collagen I in cells grown in a differentiation medium, was the highest on s6 samples. On s5 samples, coated with homogeneous TiO2 films, both cell numbers and intensity of fluorescence of collagen I was relatively high. The bone matrix mineralization, evaluated by Alizarin Red staining, was the highest on s5 samples in standard culture medium, and similar on all tested samples in differentiation medium. Thus, the surface modification of s5 samples could be considered the most suitable for application in bone implants. Keywords: Ti6-Al4-V; SAOS-2 cells; anodization; bone implants; collagen I; mineralization Available at various institutes of the ASCR
Growth and differentiation of human osteoblast-like cells on TI-6AL-4V alloy modified with anodization

Titanium and its alloys are widely used as hard tissue implants for their high biocompatibility and suitable physical properties. Along with other surface treatment methods, anodization technique is ...

Doubková, Martina; Bačáková, Lucie; Pařízek, Martin; Vandrovcová, Marta; Musílková, Jana; Lisá, Věra; Gabor, R.; Marvan, J.
Fyziologický ústav, 2015

Case study of intracranial EEG records of patients with focal cortical dysplasia type I and II
Balach, J.; Ježdík, P.; Čmejla, R.; Kršek, P.; Jiruška, Přemysl
2015 - English
In this study we try to find out if it is possible to differentiate type of focal cortical dysplasia by features obtained from intracranial EEG. We compare occurrence and rates of three biomarkers present in epilepsy in patients with focal cortical dysplasia type I and II. Case study is made on long term night records of 6 pediatric patients. Detection of interictal epileptiform discharges and high-frequency oscillations is made by automated algorithms, delta brush are marked visually. Position of lesion and electrodes inside were obtained from MRI. In individual rates were not found difference on significant level. No major significance were found, but as promising seem to be ratio inside to outside rates of high-frequency oscillations and presence of delta brush, which were found only in patients with focal cortical dysplasia type II. Keywords: intracranial EEG; focal cortical dysplasia; IED; high frequency oscillations; delta brush Available at various institutes of the ASCR
Case study of intracranial EEG records of patients with focal cortical dysplasia type I and II

In this study we try to find out if it is possible to differentiate type of focal cortical dysplasia by features obtained from intracranial EEG. We compare occurrence and rates of three biomarkers ...

Balach, J.; Ježdík, P.; Čmejla, R.; Kršek, P.; Jiruška, Přemysl
Fyziologický ústav, 2015

Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications
Kročilová, Nikola; Bačáková, Lucie; Pařízek, Martin; Havlíková, Jana; Motarjemi, H.; Molitor, M.; Gabor, R.; Marvan, J.
2015 - English
Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and proliferation activity of cells isolated under -700 mmHg (ASC-H) were higher than in cells isolated under -200 mmHg (ASC-L). However, the ASC-L was more active in osteogenic differentiation, as manifested by a higher intensity of fluorescence of alkaline phosphatase and osteocalcin in these cells. The adhesion and growth of ASCs were then studied on Ti-6Al-4V samples either unmodified (K, roughness parameter R-a = 280 nm) or modified by shot blasting and tarnishing A, R-a = 200 nm), vibratory finishing (B, R-a = 100 nm) and vibratory finishing, shot blasting and polishing (C, R-a = 80 nm). All modified samples were more wettable than the K samples. On day 1 after seeding, the size of cell spreading area on some modified samples was lower than on K samples, namely on B samples in ASC-L, on B and C in ASC-H and on A in control MG-63 cells. On day 3, the cell number on some modified samples became higher than on K samples, namely on C samples (ASC-L), on B (ASC-H) and on B and C s (MG-63 cells). On day 7, the numbers of ASC-H and MG-63 cells on all modified samples evened out and became significantly higher than on K samples. On ASC-L samples, the highest cell numbers were obtained on A samples. Thus, all studied modifications of Ti-6Al-4V enhanced growth of ASCs and human osteoblast-like MG-63 cells. Keywords: bone implants; bone tissue engineering; surface roughness; stem cells; liposuction; cell proliferation Available at various institutes of the ASCR
Growth of human adipose-derived stem cells on Ti-6Al-4V alloy with various surface modifications

Adipose-derived stem cells (ASCs) are of a great importance for bone tissue engineering. We compared ASCs obtained by liposuction under two pressures (-200 mmHg and -700 mmHg). The number and ...

Kročilová, Nikola; Bačáková, Lucie; Pařízek, Martin; Havlíková, Jana; Motarjemi, H.; Molitor, M.; Gabor, R.; Marvan, J.
Fyziologický ústav, 2015

Analysis of high-frequency activity in electroencephalogram of animal epilepsy model
Havel, T.; Kudláček, J.; Jiruška, Přemysl; Ježdík, P.; Čmejla, R.
2015 - English
High frequency oscillations (HFO) are believed to be a new specific biomarker of epileptogenic tissue. According to the reported findings, we hypothesized that HFOs occur more specific to the epileptogenic tissue. To test this hypothesis, we analyzed intracranial electroencephalograms (iEEG) of three subjects of animal epilepsy model. Overall 180 minutes of iEEG records were processed by the automatic high frequency activity detector and further analyzed. The dominant frequency of each segment was determined and categorized as ripples (80-200 Hz) or fast ripples (200-1000 Hz). Even though overall number of HFO detections predominate in the hemisphere where epileptic focus is located, in comparison of each brain structure separately our hypothesis cannot be fully confirmed. Keywords: epilepsy; animal model; electroencephalogram (EEG); high-frequency oscillations (HFO) Available at various institutes of the ASCR
Analysis of high-frequency activity in electroencephalogram of animal epilepsy model

High frequency oscillations (HFO) are believed to be a new specific biomarker of epileptogenic tissue. According to the reported findings, we hypothesized that HFOs occur more specific to the ...

Havel, T.; Kudláček, J.; Jiruška, Přemysl; Ježdík, P.; Čmejla, R.
Fyziologický ústav, 2015

Průmyslová mikrofluidizace liposomálních a hydrofobních lékových forem
Vlk, M.; Poučková, P.; Ježek, Petr
2014 - Czech
Zavedení a validace sterilní mikrofluidizace lecitinových liposomů v čistých prostorách firmy Wake s. r. o. Bude sloužit k výrobě liposomálního přípravku s ftalocyaninem pro fotodynamickou léčbu tumorů Development and validation of sterile microfluidization of lecithin liposomes in the clean facilities of company Wake s. r. o. It will serve for manufacturing of liposomal preparate with phtalocyanine for photodynamic treatment of tumors Keywords: phytodynamic therapy; phtalocyanine; microfluidization Available at various institutes of the ASCR
Průmyslová mikrofluidizace liposomálních a hydrofobních lékových forem

Zavedení a validace sterilní mikrofluidizace lecitinových liposomů v čistých prostorách firmy Wake s. r. o. Bude sloužit k výrobě liposomálního přípravku s ftalocyaninem pro fotodynamickou léčbu ...

Vlk, M.; Poučková, P.; Ježek, Petr
Fyziologický ústav, 2014

Odborná zpráva o postupu prací a dosažených výsledcích za rok 2014 v rámci projektu TA04011345
Matějková, E.; Matějka, R.; Filová, Elena
2014 - Czech
Zpráva shrnuje postupy realizace projektu TA04011345 Cévní protézy o malém průměru osídlované endotelovými a kmenovými buňkami kostní dřeně v bioreaktoru v rámci etapy 2014. Hlavními body jsou: návrh kultivační komory, izolace a charakterizace kmenových buněk, odběr cév, postupy deceluarizace cév a optimalizace polymerních nosičů pro buňky\n Report sums progress of project TA04011345. Main topics are: cultivation chamber design, isolation of stem cells, harvesting of arteries used for decellularization, methods used for decellularization and optimization of polymer surfaces Keywords: annual report; TACR Available at various institutes of the ASCR
Odborná zpráva o postupu prací a dosažených výsledcích za rok 2014 v rámci projektu TA04011345

Zpráva shrnuje postupy realizace projektu TA04011345 Cévní protézy o malém průměru osídlované endotelovými a kmenovými buňkami kostní dřeně v bioreaktoru v rámci etapy 2014. Hlavními body jsou: návrh ...

Matějková, E.; Matějka, R.; Filová, Elena
Fyziologický ústav, 2014

Funkční mapování a testování excitability limbických struktur in vivo
Kudláček, Jan; Levčík, David; Vlk, Pavel; Demeterová, Ľubica; Otáhal, Jakub; Čmejla, R.; Jiruška, Přemysl
2014 - Czech
V této práci jsme se zaměřili na analýzu funkční konektivity a excitability limbických struktur s pomocí stimulace ventrální komisury in vivo. Výsledky prokazují, že stimulace ventrální komisury aktivuje především dorzální hipokampus a mediální entorinální kůru We have focused on analysis of functional connectivity and excitability testing of limbic structures in vivo. Results demonstrated that stimulation of ventral commisure activates preferentially dorsal hippocampus and medial entorhinal cortex Keywords: limbic structures; hippocampus; entorhinal cortex; ventral commisure Available at various institutes of the ASCR
Funkční mapování a testování excitability limbických struktur in vivo

V této práci jsme se zaměřili na analýzu funkční konektivity a excitability limbických struktur s pomocí stimulace ventrální komisury in vivo. Výsledky prokazují, že stimulace ventrální komisury ...

Kudláček, Jan; Levčík, David; Vlk, Pavel; Demeterová, Ľubica; Otáhal, Jakub; Čmejla, R.; Jiruška, Přemysl
Fyziologický ústav, 2014

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